García-González María, Gómez-Bernal Fuensanta, Quevedo-Abeledo Juan C, Fernández-Cladera Yolanda, González-Rivero Agustín F, López-Mejías Raquel, Díaz-González Federico, González-Gay Miguel Á, Ferraz-Amaro Iván
Division of Rheumatology, Hospital Universitario de Canarias, 38320 Tenerife, Spain.
Division of Central Laboratory, Hospital Universitario de Canarias, 38320 Tenerife, Spain.
J Clin Med. 2023 Aug 20;12(16):5405. doi: 10.3390/jcm12165405.
Cholesterol efflux capacity (CEC), the ability of high-density lipoprotein (HDL) cholesterol to accept cholesterol from macrophages, has been linked to cardiovascular events. Systemic lupus erythematosus (SLE) is characterized by the consumption of complement (C) proteins and has been associated with an increased risk of cardiovascular disease. CEC is reduced in SLE patients compared to controls. In the present work, our objective was to analyze whether the disruption of C influences CEC in patients with SLE. New-generation functional assays of the three pathways of the C system were performed in 207 patients with SLE. Additionally, serum levels of inactive (C1q, C2, C3, C4, and factor D) and activated (C3a) molecules, and regulators (C1-inhibitor and factor H) of C system were measured. CEC, using an in vitro assay, and lipoprotein serum concentrations were assessed. Multivariable linear regression analysis was performed to assess the relationship between C system and CEC. After full multivariable analysis, the alternative C cascade functional test showed a significant and negative relationship with CEC. This was also the case for C2 and C3, in which the associations were found to be positive and statistically significant, after adjustment for covariates. In conclusion, C system and CEC are interconnected in patients with SLE.
胆固醇流出能力（CEC），即高密度脂蛋白（HDL）胆固醇从巨噬细胞摄取胆固醇的能力，与心血管事件有关。系统性红斑狼疮（SLE）的特征是补体（C）蛋白的消耗，并与心血管疾病风险增加有关。与对照组相比，SLE患者的CEC降低。在本研究中，我们的目的是分析补体系统的破坏是否会影响SLE患者的CEC。对207例SLE患者进行了补体系统三条途径的新一代功能检测。此外，还测量了补体系统的无活性（C1q、C2、C3、C4和因子D）和活化（C3a）分子以及调节因子（C1抑制因子和因子H）的血清水平。使用体外检测法评估CEC和脂蛋白血清浓度。进行多变量线性回归分析以评估补体系统与CEC之间的关系。经过全面的多变量分析后，替代补体级联功能检测显示与CEC存在显著的负相关关系。C2和C3也是如此，在对协变量进行调整后，发现它们之间的关联为正且具有统计学意义。总之，补体系统与CEC在SLE患者中相互关联。
