Morsy Menna H E, Nabil Zohour I, Darwish Samah T, Al-Eisa Rasha A, Mehana Amir E
Department of Zoology, Faculty of Science, Arish University, Arish 45511, Egypt.
Department of Zoology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt.
Life (Basel). 2023 Aug 5;13(8):1693. doi: 10.3390/life13081693.
One of the most important health issues facing the world today is obesity. It is an important independent risk factor for developing type 2 diabetes. Harmine offers various pharmacological effects, such as anti-inflammatory and anti-tumor effects. The current study aims to investigate Harmine's anti-diabetic and anti-adipogenic properties in albino mice after inducing low-grade inflammation with a high-fat diet (HFD). About forty-eight male albino mice were divided into four groups. Group 1: control mice were injected with daily saline and fed a normal chow diet of 21% protein for 5 months. Group 2: mice were treated daily with IP-injected Harmine (30 mg/kg body weight) and were fed a normal chow diet for 5 months. Group 3: mice were fed HFD to induce type 2 Diabetes Mellitus (T2DM) for 5 months. Group 4: mice were fed HFD for 14 weeks and treated with Harmine for the last 6 weeks. A figh-fat diet caused a significant increase in body and organ weight, lipid profiles, and destructive changes within the pancreas, kidney, and liver tissue. The administration of Harmine led to a remarkable improvement in the histological and ultrastructural changes induced by HFD. The findings indicate that mice cured using Harmine had lower oxidative stress, a higher total antioxidant capacity, and a reduced lipid profile compared to HFD mice. Harmine led to the hepatocytes partly restoring their ordinary configuration. Furthermore, it was noticed that the pathological incidence of damage in the structure of both the kidney and pancreas sections reduced in comparison with the diabetic group. Additional research will be required to fully understand Harmine and its preventive effects on the two forms of diabetes.
当今世界面临的最重要的健康问题之一是肥胖。它是发展为2型糖尿病的一个重要独立危险因素。骆驼蓬碱具有多种药理作用，如抗炎和抗肿瘤作用。本研究旨在探讨在高脂饮食（HFD）诱导低度炎症后，骆驼蓬碱对白化病小鼠的抗糖尿病和抗脂肪生成特性。约48只雄性白化病小鼠被分为四组。第1组：对照小鼠每天注射生理盐水，并喂食含21%蛋白质的正常饲料5个月。第2组：小鼠每天腹腔注射骆驼蓬碱（30mg/kg体重），并喂食正常饲料5个月。第3组：小鼠喂食高脂饮食以诱导2型糖尿病（T2DM）5个月。第4组：小鼠喂食高脂饮食14周，并在最后6周用骆驼蓬碱治疗。高脂饮食导致体重、器官重量、血脂水平显著增加，以及胰腺、肾脏和肝脏组织的破坏性变化。骆驼蓬碱的给药导致高脂饮食诱导的组织学和超微结构变化有显著改善。研究结果表明，与高脂饮食小鼠相比，用骆驼蓬碱治疗的小鼠氧化应激较低，总抗氧化能力较高，血脂水平降低。骆驼蓬碱使肝细胞部分恢复其正常形态。此外，还注意到与糖尿病组相比，肾脏和胰腺切片结构损伤的病理发生率降低。需要进一步的研究来充分了解骆驼蓬碱及其对两种糖尿病形式的预防作用。
