Kilany Omnia E, Abdelrazek Heba M A, Aldayel Tahany Saleh, Abdo Shimaa, Mahmoud Manal M A
Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Saudi J Biol Sci. 2020 Oct;27(10):2733-2746. doi: 10.1016/j.sjbs.2020.06.026. Epub 2020 Jun 23.
Present research explored the anti-obesity effect of seed oil extract and lycopene (LYC). Forty eight male Sprauge Dawely rats were divided equally into 6 groups. Group Ι (C) served as control, group ΙΙ (MC) was given seed oil extract (800 mg/kg b.wt) for 8 weeks, group ΙΙΙ (LC) was given (20 mg/kg b.wt) LYC for 8 weeks, group ΙV (O) received high fat diet (HFD) for 20 weeks, group Ѵ (MO), was given HFD for 20 weeks and received (800 mg/kg b.wt) seed oil extract for last 8 weeks and group ѴΙ (LO), received HFD for 20 weeks and was given (20 mg/kg b.wt) LYC for last 8 weeks. Hematology, lipid peroxidation and antioxidants, non-esterified fatty acids (NEFA), glucose, lipid profile, serum liver and kidney biomarkers, inflammatory markers, leptin, resistin and heart fatty acid binding protein (HFABP) were determined. Also histopathology for liver, kidney and aorta were performed besides immunohistochemistry (IHC) for aortic inducible nitric oxide synthase (iNOS). Administration of seed oil extract and LYC significantly ameliorated the HFD induced hematological and metabolic perturbations as well as reduced leptin and resistin. Both treatments exerted these effects through promotion of antioxidant enzymes and reducing lipid peroxidation as well as inflammatory cytokines along with reduced iNOS protein expression. Administration of seed oil extract and LYC have anti-obesity potential in HFD induced obesity in male Sprauge Dawely rats.
目前的研究探讨了籽油提取物和番茄红素（LYC）的抗肥胖作用。48只雄性Sprauge Dawely大鼠被平均分为6组。第一组（C）作为对照组，第二组（MC）给予籽油提取物（800mg/kg体重），持续8周；第三组（LC）给予（20mg/kg体重）LYC，持续8周；第四组（O）接受高脂饮食（HFD）20周；第五组（MO）接受HFD 20周，并在最后8周给予（800mg/kg体重）籽油提取物；第六组（LO）接受HFD 20周，并在最后8周给予（20mg/kg体重）LYC。测定了血液学指标、脂质过氧化和抗氧化剂、非酯化脂肪酸（NEFA）、葡萄糖、血脂谱、血清肝肾功能生物标志物、炎症标志物、瘦素、抵抗素和心脏脂肪酸结合蛋白（HFABP）。此外，还进行了肝脏、肾脏和主动脉的组织病理学检查以及主动脉诱导型一氧化氮合酶（iNOS）的免疫组织化学（IHC）检查。给予籽油提取物和LYC可显著改善HFD诱导的血液学和代谢紊乱，并降低瘦素和抵抗素水平。两种治疗均通过促进抗氧化酶、减少脂质过氧化以及炎症细胞因子，同时降低iNOS蛋白表达来发挥这些作用。在雄性Sprauge Dawely大鼠中，给予籽油提取物和LYC对HFD诱导的肥胖具有抗肥胖潜力。
