Kemerovo State University, Kemerovo, Russia.
Braz J Biol. 2022 Mar 14;84:e256944. doi: 10.1590/1519-6984.256944. eCollection 2022.
In order to ensure the timely and uninterrupted supply of medicinal plant raw materials, the methods of cultivation of plant cell cultures, namely, the production of plant root cultures, are relevant. In this paper, the geroprotective potential of Hedysarum neglectum Ledeb and Panax ginseng C. A. Mey root cultures is studied. They were cultured under in vitro conditions by transforming the rhizome (H. neglectum) and seed seedlings (P. ginseng) with Agrobacterium rhizogenes 15834 Swiss. To identify the geroprotective potential, the antimicrobial disc-diffusion method and the antioxidant activity were analyzed by titration of KMnO4 extracts of plant root cultures. The qualitative and quantitative composition was analyzed using high-performance liquid chromatography, thin-layer chromatography, and gas chromatography with mass spectrometry. In the course of the work, the presence of antimicrobial and antioxidant activity of plant root culture extracts was established. Biologically active substances contained in extracts of Hedysarum neglectum Ledeb root crops and Panax ginseng C. A. Mey are characterized by geroprotective potential, so they can act as a source of natural antioxidants in the functional nutrition of the geroprotective orientation.
为了确保药用植物原料的及时和不间断供应，培养植物细胞培养物的方法，即植物根培养物的生产，是相关的。本文研究了甘草根培养物和人参根培养物的抗衰老潜力。通过用根癌农杆菌 15834 瑞士转化根茎（甘草）和种子幼苗（人参），在体外条件下培养它们。为了鉴定抗衰老潜力，通过滴定植物根培养物的 KMnO4 提取物分析了抗菌圆盘扩散法和抗氧化活性。使用高效液相色谱法、薄层层析法和气相色谱法与质谱联用分析了定性和定量组成。在工作过程中，确立了植物根培养物提取物的抗菌和抗氧化活性。甘草根作物和人参 C. A. 根提取物中含有的生物活性物质具有抗衰老潜力，因此它们可以作为具有抗衰老方向的功能性营养天然抗氧化剂的来源。
