Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Biosensors (Basel). 2021 Sep 23;11(10):351. doi: 10.3390/bios11100351.
This work describes the facile preparation of a disposable electrochemical biosensor for the detection of Hg(II) in water by modifying the surface of a screen-printed carbon electrode (SPCE). The surface modification consists of the immobilization of a composite layer of silver nanowires, hydroxymethyl propyl cellulose, chitosan, and urease (AgNWs/HPMC/CS/Urease). The presence of the composite was confirmed by scanning electron microscopy (SEM) and its excellent conductivity, due chiefly to the electrical properties of silver nanowires, enhanced the sensitivity of the biosensor. Under optimum conditions, the modified SPCE biosensor showed excellent performance for the detection of Hg(II) ions, with an incubation time of 10 min and a linear sensitivity range of 5-25 µM. The limit of detection (LOD) and limit of quantitation (LOQ) were observed to be 3.94 µM and 6.50 µM, respectively. In addition, the disposable and portable biosensor exhibited excellent recoveries for the detection of Hg(II) ions in commercial drinking water samples (101.62-105.26%). The results are correlated with those obtained from inductively coupled plasma optical emission spectrometry (ICP-OES), indicating that our developed sensor is a reliable method for detection of Hg(II) in real water samples. The developed sensor device is a simple, effective, portable, low cost, and user-friendly platform for real-time detection of heavy metal ions in field measurements with potential for other biomedical applications in the future.
这项工作描述了一种通过修饰丝网印刷碳电极（SPCE）表面来制备用于检测水中 Hg(II) 的一次性电化学生物传感器的简便方法。表面修饰包括固定化银纳米线、羟甲基纤维素、壳聚糖和脲酶的复合层（AgNWs/HPMC/CS/Urease）。通过扫描电子显微镜（SEM）证实了复合材料的存在，由于银纳米线的电性能，其优异的导电性增强了生物传感器的灵敏度。在最佳条件下，修饰后的 SPCE 生物传感器对 Hg(II) 离子的检测表现出优异的性能，孵育时间为 10 分钟，线性灵敏度范围为 5-25µM。检测限（LOD）和定量限（LOQ）分别观察到为 3.94µM 和 6.50µM。此外，这种一次性和便携式生物传感器在商业饮用水样品中对 Hg(II) 离子的检测表现出优异的回收率（101.62-105.26%）。结果与电感耦合等离子体发射光谱法（ICP-OES）获得的结果相关，表明我们开发的传感器是一种用于检测实际水样中 Hg(II) 的可靠方法。该开发的传感器设备是一种简单、有效、便携、低成本且用户友好的平台，可用于现场测量重金属离子的实时检测，并具有未来在其他生物医学应用中的潜力。
