Touzout Soraya Naila, Merghni Abderrahmen, Laouani Aicha, Boukhibar Halima, Alenazy Rawaf, Alobaid Abdulmohsen, Alenazy Mustafa, Ben-Attia Mossadok, Saguem Khaled, El-Bok Safia
Laboratory of Biodiversity, Biotechnologies and Climate Change (LR11/ES09), Faculty of Sciences of Tunis, University of Tunis El-Manar, Tunis 2092, Tunisia.
Laboratory of Antimicrobial Resistance LR99ES09, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia.
Microorganisms. 2023 Aug 11;11(8):2062. doi: 10.3390/microorganisms11082062.
L., a family tree, is widely used in traditional folkloric medicine for its pharmaceutical properties. In the present study, we investigated the phytochemical composition of four methanolic leaf extracts of of various origins (Algeria and Tunisia) using high-performance liquid chromatography (HPLC). The antibacterial efficacy and mechanisms of action against Gram-positive and Gram-negative pathogenic microorganisms were then evaluated. Our findings revealed a presence of phenolic acids and flavonoids, such as gallic acid, chlorogenic acid, caffeic acid, hyperoside, isoquercetin, quercetin, and isorhamnetin both in Algerian and Tunisian localities, with an abundance of phenolic acids compared to flavonoids. Additionally, the studied extracts exhibit a broad spectrum of antibacterial activities, with MIC values ranging from 31.25 mg/mL to 125 mg/mL. Methanolic leaf extracts of from Algeria exhibited more potent biofilm eradication, with a percentage of inhibition reaching 72.17% against the strain. Furthermore, inhibitory concentrations of tested substances, particularly the extract from the Relizane area, were capable of disrupting the membrane integrity of the treated bacteria as well as producing oxidative stress through ROS generation. Likewise, our results reveal that plant extract induces lipid peroxidation by raising MDA levels in comparison to untreated cells, particularly with the plant extract of Blida. extracts also reduced the synthesis of antioxidant enzymes (CAT and SOD). Our findings illustrate that remains a plant with significant antibacterial potential and distinct mechanisms of action that are closely related to the origins of this specimen.
L.，一种植物，因其药用特性在传统民间医学中被广泛使用。在本研究中，我们使用高效液相色谱法（HPLC）研究了来自不同产地（阿尔及利亚和突尼斯）的四种L.叶甲醇提取物的植物化学成分。然后评估了其对革兰氏阳性和革兰氏阴性致病微生物的抗菌功效及作用机制。我们的研究结果显示，在阿尔及利亚和突尼斯产地的提取物中均存在酚酸和黄酮类化合物，如没食子酸、绿原酸、咖啡酸、金丝桃苷、异槲皮苷、槲皮素和异鼠李素，与黄酮类化合物相比，酚酸含量更为丰富。此外，所研究的提取物表现出广泛的抗菌活性，最低抑菌浓度（MIC）值范围为31.25毫克/毫升至125毫克/毫升。来自阿尔及利亚的L.叶甲醇提取物表现出更强的生物膜消除能力，对某菌株的抑制率达到72.17%。此外，受试物质的抑制浓度，特别是来自雷利赞地区的提取物，能够破坏被处理细菌的膜完整性，并通过活性氧（ROS）的产生引发氧化应激。同样，我们的结果表明，与未处理的细胞相比，植物提取物通过提高丙二醛（MDA）水平诱导脂质过氧化，特别是Blida地区的植物提取物。L.提取物还降低了抗氧化酶（过氧化氢酶和超氧化物歧化酶）的合成。我们的研究结果表明，L.仍然是一种具有显著抗菌潜力且作用机制独特的植物，这些机制与该样本的产地密切相关。
