Liu Xuyan, Zhu Xishen, Dong Yumei, Chen Yan, Li Meifang, Li Chengyun
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
Microorganisms. 2023 Aug 15;11(8):2085. doi: 10.3390/microorganisms11082085.
Soil has been considered the main microbial reservoir for plants, but the robustness of the plant microbiome when the soil resource is removed has not been greatly considered. In the present study, we tested the robustness of the microbiota recruited by Tartary buckwheat ( Gaertn.), grown on sterile humus soil and irrigated with sterile water. Our results showed that the microbiomes of the leaf, stem, root and next-generation seeds were comparable between treated (grown in sterile soil) and control plants (grown in non-sterile soil), indicating that the plants had alternative robust ways to shape their microbiome. Seed microbiota contributed greatly to endophyte communities in the phyllosphere, rhizosphere and next-generation seeds. The microbiome originated from the seeds conferred clear benefits to seedling growth because seedling height and the number of leaves were significantly increased when grown in sterilized soil. The overall microbiome of the plant was affected very little by the removal of the soil microbial resource. The microbial co-occurrence network exhibited more interactions, and Proteobacteria was enriched in the root of Tartary buckwheat planted in sterilized soil. Our research broadens the understanding of the general principles governing microbiome assembly and is widely applicable to both microbiome modeling and sustainable agriculture.
土壤一直被认为是植物的主要微生物库，但当土壤资源被去除时，植物微生物组的稳健性尚未得到充分考虑。在本研究中，我们测试了在无菌腐殖土上生长并用无菌水灌溉的苦荞麦（Gaertn.）所招募的微生物群的稳健性。我们的结果表明，处理组（在无菌土壤中生长）和对照组植物（在非无菌土壤中生长）的叶、茎、根和下一代种子的微生物组具有可比性，这表明植物有其他稳健的方式来塑造其微生物组。种子微生物群对叶际、根际和下一代种子中的内生菌群落有很大贡献。源自种子的微生物组对幼苗生长有明显益处，因为在无菌土壤中生长时，幼苗高度和叶片数量显著增加。去除土壤微生物资源对植物的整体微生物组影响很小。微生物共现网络显示出更多的相互作用，并且变形菌门在无菌土壤中种植的苦荞麦根中富集。我们的研究拓宽了对微生物组组装一般原则的理解，并且广泛适用于微生物组建模和可持续农业。
