State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2023 Aug 21;15(16):3659. doi: 10.3390/nu15163659.
Microbiota-derived desaminotyrosine (DAT) protects the host from influenza by modulating the type I interferon (IFN) response. The aim of this study was to investigate the antivirus effects of a DAT-producing bacteria strain. A comparative genomics analysis and UHPLC Q-Exactive MS were used to search for potential strains and confirm their ability to produce DAT, respectively. The anti-influenza functions of the DAT producer were evaluated using an antibiotic-treated mouse model by orally administering the specific strain before viral infection. The results showed the CCFM1227 contained the gene and produced DAT by degrading phloretin. In vivo, CCFM1227 re-inoculation increased the DAT level in feces, and protected from influenza through inhibiting viral replication and alleviating lung immunopathology. Furthermore, CCFM1227-derived DAT was positively correlated with the IFN-β level in the lung. The transcriptome results showed that CCFM1227 activated gene expression in the context of the defense response to the virus, and the response to interferon-beta. Moreover, CCFM1227 treatment upregulated the expression of MHC-I family genes, which regulate the adaptive immune response. In conclusion, CCFM1227 exerted antiviral effects by producing DAT in the gut, and this may provide a potential solution for creating effective antiviral probiotics.
肠道微生物衍生的去氨酪氨酸（DAT）通过调节 I 型干扰素（IFN）反应来保护宿主免受流感的侵害。本研究旨在研究产 DAT 细菌菌株的抗病毒作用。采用比较基因组学分析和 UHPLC Q-Exactive MS 分别用于寻找潜在的菌株并确认其产生 DAT 的能力。通过在病毒感染前口服给予特定菌株，使用抗生素处理的小鼠模型评估 DAT 产生菌的抗流感功能。结果表明，CCFM1227 含有 datA 基因，并通过降解根皮苷产生 DAT。在体内，CCFM1227 的再接种增加了粪便中的 DAT 水平，并通过抑制病毒复制和减轻肺部免疫病理学来预防流感。此外，CCFM1227 衍生的 DAT 与肺部 IFN-β 水平呈正相关。转录组结果表明，CCFM1227 在针对病毒的防御反应和干扰素-β反应中激活了基因表达。此外，CCFM1227 处理上调了 MHC-I 家族基因的表达，这些基因调节适应性免疫反应。总之，CCFM1227 通过在肠道中产生 DAT 发挥抗病毒作用，这可能为开发有效的抗病毒益生菌提供了一种潜在的解决方案。
