Jiang Kangjia, Wang Duo, Su Le, Liu Xinli, Yue Qiulin, Zhang Song, Zhao Lin
State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Shandong Chenzhang Biotechnology Co., Ltd., Jinan 250353, China.
Pharmaceuticals (Basel). 2023 Aug 10;16(8):1133. doi: 10.3390/ph16081133.
(1) Background: Ulcerative colitis (UC) is a disease caused by noninfectious chronic inflammation characterized by varying degrees of inflammation affecting the colon or its entire mucosal surface. Current therapeutic strategies rely on the suppression of the immune response, which is effective, but can have detrimental effects. Recently, different plant polysaccharides and their degradation products have received increasing attention due to their prominent biological activities. The aim of this research was to evaluate the mitigation of inflammation exhibited by tamarind seed polysaccharide hydrolysate (TSPH) ingestion in colitis mice. (2) Methods: TSPH was obtained from the hydrolysis of tamarind seed polysaccharide (TSP) by trifluoroacetic acid (TFA). The structure and physical properties of TSPH were characterized by ultraviolet spectroscopy (UV), thin-layer chromatography (TLC), fourier transform infrared spectroscopy (FT-IR), and High-Performance Liquid Chromatography and Electrospray Ionization Mass Spectrometry (HPLC-ESI/MS) analysis. Then, the alleviative effects of the action of TSPH on 2.5% dextran sodium sulfate (DSS)-induced colitis mice were investigated. (3) Results: TSPH restored pathological lesions in the colon and inhibited the over-secretion of pro-inflammatory cytokines in UC mice. The relative expression level of mRNA for colonic tight junction proteins was increased. These findings suggested that TSPH could reduce inflammation in the colon. Additionally, the structure of the gut microbiota was also altered, with beneficial bacteria, including and , significantly enriched by TSPH. Moreover, the richness of was positively correlated with acetic acid. (4) Conclusions: In conclusion, TSPH suppressed colonic inflammation, alleviated imbalances in the intestinal flora and regulated bacterial metabolites. Thus, this also implies that TSPH has the potential to be a functional food against colitis.
(1) 背景：溃疡性结肠炎（UC）是一种由非感染性慢性炎症引起的疾病，其特征是结肠或整个黏膜表面出现不同程度的炎症。目前的治疗策略依赖于免疫反应的抑制，这虽然有效，但可能会产生有害影响。最近，不同的植物多糖及其降解产物因其显著的生物活性而受到越来越多的关注。本研究的目的是评估摄入罗望子种子多糖水解物（TSPH）对结肠炎小鼠炎症的缓解作用。(2) 方法：通过三氟乙酸（TFA）水解罗望子种子多糖（TSP）获得TSPH。通过紫外光谱（UV）、薄层色谱（TLC）、傅里叶变换红外光谱（FT-IR）以及高效液相色谱和电喷雾电离质谱（HPLC-ESI/MS）分析对TSPH的结构和物理性质进行表征。然后，研究TSPH对2.5%葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的缓解作用。(3) 结果：TSPH恢复了结肠的病理损伤，并抑制了UC小鼠促炎细胞因子的过度分泌。结肠紧密连接蛋白的mRNA相对表达水平升高。这些发现表明TSPH可以减轻结肠炎症。此外，肠道微生物群的结构也发生了改变，TSPH使包括[具体有益菌1]和[具体有益菌₂]在内的有益菌显著富集。而且，[具体有益菌]的丰度与乙酸呈正相关。(4) 结论：总之，TSPH抑制了结肠炎症，缓解了肠道菌群失衡并调节了细菌代谢产物。因此，这也意味着TSPH有潜力成为一种抗结肠炎的功能性食品。
