Zhang Jie, Zhang Chunyan, Xue Hongdan, Lu Chengbo, Rong Rong, Li Jinjing, Zhou Shujing
Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China.
HeBei University of Architecture, Zhangjiakou 075000, China.
Pharmaceuticals (Basel). 2023 Aug 10;16(8):1135. doi: 10.3390/ph16081135.
A new PES-C/emodin ultrafiltration membrane was prepared by blending natural emodin with phenolphthalein polyethersulfone (PES-C) and was used to purify an acanthopanax senticosus injection in this study. Regarding the purified acanthopanax senticosus injection, its color became lighter, and its clarity increased. On the contrary, for an acanthopanax senticosus injection containing macromolecules, its color deepened, and its turbidity increased. Thermal stability of the purified acanthopanax senticosus injection was the best, followed by the original solution of the acanthopanax senticosus injection, and the acanthopanax senticosus injection containing macromolecules was the worst. The fingerprint spectrum of the purified acanthopanax senticosus injection was similar to the original solution of the acanthopanax senticosus injection, the relative peak area of each single peak was greater than 0.95, and the relative peak area of the total peak was greater than 0.96. Compared with the original solution of the acanthopanax senticosus injection, the histamine release amount and cell degranulation rate of the acanthopanax senticosus injection containing macromolecules increased, while those of the purified acanthopanax senticosus injection decreased, which reduced the risk of allergic reaction to a certain extent. "Inverse proof" confirmed that the acanthopanax senticosus injection containing macromolecules had certain liver and kidney toxicity, which indirectly proved that the liver and kidney toxicity of the purified acanthopanax senticosus injection was effectively reduced.
通过将天然大黄素与酚酞聚醚砜（PES-C）共混制备了一种新型的PES-C/大黄素超滤膜，并在本研究中用于纯化刺五加注射液。对于纯化后的刺五加注射液，其颜色变浅，澄清度提高。相反，对于含有大分子的刺五加注射液，其颜色加深，浊度增加。纯化后的刺五加注射液热稳定性最佳，其次是刺五加注射液原液，含有大分子的刺五加注射液热稳定性最差。纯化后的刺五加注射液指纹图谱与刺五加注射液原液相似，各单峰相对峰面积大于0.95，总峰相对峰面积大于0.96。与刺五加注射液原液相比，含有大分子的刺五加注射液组胺释放量和细胞脱颗粒率增加，而纯化后的刺五加注射液组胺释放量和细胞脱颗粒率降低，这在一定程度上降低了过敏反应风险。“反证”证实含有大分子的刺五加注射液具有一定的肝肾毒性，间接证明纯化后的刺五加注射液肝肾毒性有效降低。
