Karstarli Bakay Ozge Sevil, Demir Betül, Cicek Demet, Erol Deniz, Aşçı Toraman Zulal, Gural Yunus, Maurer Marcus
Department of Dermatology, Pamukkale University Faculty of Medicine, Denizli, Turkey.
Department of Dermatology, Firat University Faculty of Medicine, Elazig, Turkey.
Clin Transl Allergy. 2023 Aug;13(8):e12290. doi: 10.1002/clt2.12290.
Chronic spontaneous urticaria (CSU) is a common and disabling disease. Assessments of IgE and C-reactive protein (CRP) are recommended in the diagnostic work-up, but the role and clinical relevance of these biomarkers are not well characterized. Moreover, it remains unknown if elevated levels of IgE or CRP are linked to CSU microRNA (miRNA) signatures or interleukin 31 (IL-31).
We measured IgE and CRP serum levels in 47 CSU patients (and 45 healthy controls) and determined CSU disease activity using the urticaria activity score (UAS7). Expression levels of miR-155 and miR-221 were assessed by RT-PCR, and IL-31 levels were determined by ELISA.
Total IgE and CRP levels were independently increased in CSU patients. IgE and CRP levels were highest and lowest in patients with high and mild disease activity. IgE levels correlated with miR-155 levels, whereas CRP levels correlated with miR-221 levels. miR-155 and miR-221 were significantly overexpressed in CSU patients. ROC analyses linked miRNA-155 and CSU with a sensitivity of 79% and specificity of 87%, and miRNA-221 and CSU with a sensitivity of 75% and specificity of 91%. High CRP and miR-221 expression levels were linked to elevated levels of IgG anti-TPO and IL-31.
IgE and CRP are useful biomarkers for disease activity in CSU, with distinct miRNA profiles. High CRP and miR-221 levels may point to autoimmune CSU and a role for IL-31.
慢性自发性荨麻疹（CSU）是一种常见且使人衰弱的疾病。在诊断检查中推荐评估IgE和C反应蛋白（CRP），但这些生物标志物的作用和临床相关性尚未得到充分表征。此外，IgE或CRP水平升高是否与CSU微小RNA（miRNA）特征或白细胞介素31（IL-31）相关仍不清楚。
我们测量了47例CSU患者（以及45例健康对照）的IgE和CRP血清水平，并使用荨麻疹活动评分（UAS7）确定CSU疾病活动度。通过RT-PCR评估miR-155和miR-221的表达水平，并通过ELISA测定IL-31水平。
CSU患者的总IgE和CRP水平独立升高。疾病活动度高和低的患者中IgE和CRP水平分别最高和最低。IgE水平与miR-155水平相关，而CRP水平与miR-221水平相关。miR-155和miR-221在CSU患者中显著过表达。ROC分析显示miRNA-155与CSU相关，敏感性为79%，特异性为87%，miRNA-221与CSU相关，敏感性为75%，特异性为91%。高CRP和miR-221表达水平与IgG抗甲状腺过氧化物酶和IL-31水平升高有关。
IgE和CRP是CSU疾病活动度的有用生物标志物，具有不同的miRNA谱。高CRP和miR-221水平可能提示自身免疫性CSU以及IL-31的作用。
