Department of Biosciences, COMSATS University Islamabad, Pakistan.
Department of Biosciences, COMSATS University Islamabad, Pakistan.
Plant Physiol Biochem. 2023 Sep;202:107909. doi: 10.1016/j.plaphy.2023.107909. Epub 2023 Aug 16.
Green nanobiotechnology and beneficial bacterial strains as biofertilizers are crucial in agriculture to achieve food security. Both these strategies have been individually studied in improving plant resistance against phytopathogens along with enhancing plant productivity. Therefore, objective of this study was to explore the eco-friendly and cost-effective approach of utilizing plant growth promoting and disease suppressing bacterial strains and nanoparticles, individually as well as in combination, as bio-stimulants to improve plant growth, antioxidant defense system, nutrition and yield of tomato. A pot experiment was conducted to investigate the zinc sulfide nanoparticles (ZnS NPs) synthesized by using Jacaranda mimosifolia flower extracts (JFE), Acinetobacter pittii and Bacillus velezensis either individually or in combinations to check their potential against Rhizoctonia solani in tomato to suppress root rot infection and improve growth and yield. Among all the combinations the JFE-ZnS NPs + B. velezensis compared to untreated infected plants showed minimum disease incidence and maximum significant protection (66%) against R. solani instigated root rot that was followed by JFE-ZnS NPs + A. pittii and individual application of JFE-ZnS NPs by 58%. The same treatment showed maximum significant increase in plant fresh and dry biomass. B. velezensis significantly increased the photosynthetic pigments when applied individually. However, JFE-ZnS NPs alone and in mixed treatments with B. velezensis efficiently improved total soluble protein, sugar and phenolic contents. The same interactive application of JFE-ZnS NPs + B. velezensis improved the tomato plant nutrition (silicon (Si), magnesium (Mg), calcium (Ca) and potassium (K)) and redox quenching status by improving the activity of antioxidant defense enzymes. Overall, the interactive use of JFE-ZnS NPs with A. pittii and B. velezensis very appropriately prepared the host plant to fight against the negative effects of root rot pathogen in tomato. Advancements in interactively investigating the nanoparticles with beneficial plant growth promoting bacterial strains importantly can contribute in resolving the challenges of food security. According to our information, this is a pioneer report for implying JFE-ZnS NPs in synergism with A. pittii and B. velezensis to hinder the root rot in tomatoes.
绿色纳米生物技术和有益细菌菌株作为生物肥料在农业中对于实现粮食安全至关重要。这两种策略都分别在提高植物对植物病原菌的抗性和提高植物生产力方面进行了研究。因此，本研究的目的是探索利用植物生长促进和抑制病原菌的细菌菌株和纳米颗粒的环保和经济有效的方法，单独使用或组合使用作为生物刺激剂，以提高番茄的生长、抗氧化防御系统、营养和产量。进行了一项盆栽试验，以研究使用 Jacaranda mimosifolia 花提取物 (JFE)、Acinetobacter pittii 和 Bacillus velezensis 合成的硫化锌纳米颗粒 (ZnS NPs)，单独或组合使用，以检查它们对番茄中 Rhizoctonia solani 的潜在抑制作用，以抑制根腐病感染并提高生长和产量。在所有组合中，与未处理感染植物相比，JFE-ZnS NPs + B. velezensis 表现出最低的发病率和最高的显著保护 (66%)，可抵抗 R. solani 引起的根腐病，其次是 JFE-ZnS NPs + A. pittii 和单独应用 JFE-ZnS NPs 的 58%。相同的处理表现出最大的显著增加植物鲜重和干重。B. velezensis 单独使用时可显著增加光合色素。然而，JFE-ZnS NPs 单独使用以及与 B. velezensis 的混合处理可有效提高总可溶性蛋白质、糖和酚类物质的含量。JFE-ZnS NPs + B. velezensis 的这种相互作用的应用可通过提高抗氧化防御酶的活性来改善番茄植物的营养 (硅 (Si)、镁 (Mg)、钙 (Ca) 和钾 (K)) 和氧化还原猝灭状态。总的来说，JFE-ZnS NPs 与 A. pittii 和 B. velezensis 的相互作用使用非常适当地使宿主植物为对抗番茄根腐病病原菌的负面影响做好准备。有益的植物生长促进细菌菌株与纳米颗粒相互作用的研究进展对于解决粮食安全挑战非常重要。据我们所知，这是首例将 JFE-ZnS NPs 与 A. pittii 和 B. velezensis 协同作用用于抑制番茄根腐病的报告。
