Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
School of Public Health, North China University of Science and Technology, No.21 Bohai Road, Caofeidian, Tangshan 063210, Hebei, China.
Food Chem. 2024 Jan 30;432:137208. doi: 10.1016/j.foodchem.2023.137208. Epub 2023 Aug 22.
This work proposed an in-situ blocking strategy for improved anti-interference and signal-amplified inspection of hazards via constructing hollow covalent organic framework (HCOF) capsules. An aptamer-FRET nanoprobe integrated with carbon dots and CuS was introduced into the micro-capsule as signal indicator to demonstrate the proof-of-concept. The HCOF was successfully prepared by removing the metal-organic frameworks (MOF) core from the MOF@COF that had been preloaded with the nanoprobes under mild conditions. Meanwhile, the hydrophobic surface of HCOF enhanced the adsorption and penetration of aflatoxin B1 (AFB1) into the capsule to interact with the nanoprobes. This strategy was applied to detect AFB1 in food samples, achieving a linear response of 1-300 nM along with a detection limit of 0.3 nM. Selectivity test verified that the prepared sensing platform could specifically recognize AFB1 without complex sample pretreatment. This study provides new ideas for improved anti-interference inspection of hazards against complex sample matrix.
这项工作提出了一种原位阻断策略，通过构建中空共价有机框架（HCOF）胶囊来提高抗干扰和信号放大检测危害的能力。将适体-FRET 纳米探针与碳点和 CuS 集成到微胶囊中作为信号指示剂，以验证其概念验证。HCOF 是通过在温和条件下从已经预装载有纳米探针的 MOF@COF 中去除金属-有机骨架（MOF）核心来成功制备的。同时，HCOF 的疏水性表面增强了黄曲霉毒素 B1（AFB1）进入胶囊与纳米探针相互作用的吸附和渗透。该策略用于检测食品样品中的 AFB1，实现了 1-300 nM 的线性响应，检测限为 0.3 nM。选择性测试验证了制备的传感平台可以特异性识别 AFB1，而无需复杂的样品预处理。这项研究为改进针对复杂样品基质的危害的抗干扰检测提供了新的思路。
