一种新的反相高效液相色谱-电喷雾质谱法测定维生素 E 的 8 种生育酚形式。

A new RPLC-ESI-MS method for the determination of eight vitamers of vitamin E.

机构信息

Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.

出版信息

Food Chem. 2024 Jan 30;432:137161. doi: 10.1016/j.foodchem.2023.137161. Epub 2023 Aug 15.

DOI:10.1016/j.foodchem.2023.137161

Abstract

Vitamin E consists of four (α-, β-, γ-, δ-) isoforms of tocopherols (T) and tocotrienols (T3), collectively known as tocols. Current LC methods for tocols suffer from either the poor ability to resolve the β- and γ- isoforms (RPLC), or require the use of nonpolar solvents (NPLC), which complicates subsequent MS/MS detection. Moreover, we show that coupling of UV with MS leads to tocols photodegradation. To solve these problems, we developed a new RPLC-MS/MS method, allowing to resolve not only α- and δ-, but also β- and γ- tocols in hydrophobic matrices. We took advantage of an observation that the peak area ratios are specific for the given isomer and constant. The new method with a linear range between 0.2 and 60 ng·mL (for α-T) and 1.1-60 ng·mL (for β-T3 and γ-T3) was validated and employed for quantitative analysis of several oils, including false flax (Camelina sativa) oil stored under different conditions.

摘要

维生素 E 由四种（α-、β-、γ-、δ-）生育酚（T）和三烯生育酚（T3）异构体组成，统称为生育酚。目前用于生育酚的 LC 方法要么对 β-和 γ-异构体的分辨率很差（RPLC），要么需要使用非极性溶剂（NPLC），这使得随后的 MS/MS 检测复杂化。此外，我们表明，将 UV 与 MS 耦合会导致生育酚光降解。为了解决这些问题，我们开发了一种新的 RPLC-MS/MS 方法，允许在疏水性基质中不仅分离 α-和 δ-，还分离 β-和 γ-生育酚。我们利用了一个观察结果，即峰面积比对于给定的异构体是特定的且恒定的。该新方法的线性范围为 0.2-60ng·mL（用于 α-T）和 1.1-60ng·mL（用于 β-T3 和 γ-T3），已得到验证，并用于几种油的定量分析，包括在不同条件下储存的假亚麻籽油（Camelina sativa）。