• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

评价急性白血病患者 CD8+ T 细胞中 CD244 及其衔接分子的 mRNA 表达。

Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8+ T Cells in Acute Leukemia.

机构信息

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Iran Biomed J. 2023 Jul 1;27(4):214-8. doi: 10.61186/ibj.3843.

DOI:10.61186/ibj.3843
PMID:37634081
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10507292/
Abstract

BACKGROUND

This study investigated the role of the immune-checkpoint receptor (ICR), CD244, and its adapter molecules, in CD8+ T cells in acute leukemia.

METHODS

Blood samples were obtained from 21 acute lymphoblastic leukemia (ALL) and 6 acute myeloid leukemia (AML) patients and 20 control subjects. Relative gene expression of CD244, immune receptor tyrosine-based switch motif-associated protein (SA), EWS/FLI1-activated transcript 2 (EAT-2), and LncRNA-GSTT1-AS1 were evaluated using quantitative reverse transcription polymerase chain reaction.

RESULTS

Expression of CD244, SAP, and EAT-2 were significantly lower in CD8+ T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects.

CONCLUSION

The low SAP/CD244 expression ratio in CD8+ T cells in ALL suggests an inhibitory role for CD244 in ALL.

摘要

背景

本研究探讨了免疫检查点受体（ICR）CD244 及其衔接分子在急性白血病 CD8+T 细胞中的作用。

方法

采集 21 例急性淋巴细胞白血病（ALL）、6 例急性髓细胞白血病（AML）患者和 20 例对照者的血样。采用实时定量逆转录聚合酶链反应检测 CD244、免疫受体酪氨酸基开关基序相关蛋白（SAP）、EWS/FLI1 激活转录因子 2（EAT-2）和 LncRNA-GSTT1-AS1 的相对基因表达。

结果

与对照组相比，ALL 患者 CD8+T 细胞中 CD244、SAP 和 EAT-2 的表达明显降低。有趣的是，SAP 的表达明显低于 CD244，表明 SAP 与 CD244 的比值较低。此外，与对照组相比，AML 患者 SAP 的表达显著降低。与对照组相比，ALL 和 AML 患者 LncRNA-GSTT1-AS1 的表达无明显差异。

结论

ALL 患者 CD8+T 细胞中 SAP/CD244 表达比值较低，提示 CD244 在 ALL 中起抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f46/10507292/accd8d1cbdef/ibj-27-214-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f46/10507292/29dc86d05fff/ibj-27-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f46/10507292/accd8d1cbdef/ibj-27-214-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f46/10507292/29dc86d05fff/ibj-27-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f46/10507292/accd8d1cbdef/ibj-27-214-g002.jpg

相似文献

1
Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8+ T Cells in Acute Leukemia.评价急性白血病患者 CD8+ T 细胞中 CD244 及其衔接分子的 mRNA 表达。
Iran Biomed J. 2023 Jul 1;27(4):214-8. doi: 10.61186/ibj.3843.
2
Long noncoding RNA derived from CD244 signaling epigenetically controls CD8+ T-cell immune responses in tuberculosis infection.源自CD244信号通路的长链非编码RNA通过表观遗传调控结核感染中CD8 + T细胞的免疫反应。
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3883-92. doi: 10.1073/pnas.1501662112. Epub 2015 Jul 6.
3
Increased TOX expression concurrent with PD-1, Tim-3, and CD244 expression in T cells from patients with acute myeloid leukemia.急性髓系白血病患者T细胞中TOX表达增加，同时伴有PD-1、Tim-3和CD244表达。
Cytometry B Clin Cytom. 2022 Mar;102(2):143-152. doi: 10.1002/cyto.b.22049. Epub 2021 Dec 16.
4
Scrutinizing the Expression and Blockade of Inhibitory Molecules Expressed on T Cells from Acute Myeloid Leukemia Patients.对急性髓系白血病患者T细胞上表达的抑制性分子的表达及阻断进行研究。
Iran J Allergy Asthma Immunol. 2018 Jun;17(3):265-273.
5
Increasing Tim-3+CD244+, Tim-3+CD57+, and Tim-3+PD-1+ T cells in patients with acute myeloid leukemia.急性髓系白血病患者中Tim-3+CD244+、Tim-3+CD57+和Tim-3+PD-1+ T细胞数量增加。
Asia Pac J Clin Oncol. 2020 Jun;16(3):137-141. doi: 10.1111/ajco.13304. Epub 2020 Feb 7.
6
Analysis of immune activation and clinical events in acute infectious mononucleosis.急性传染性单核细胞增多症中免疫激活与临床事件的分析。
J Infect Dis. 2004 Jul 1;190(1):63-71. doi: 10.1086/421276. Epub 2004 Jun 8.
7
Higher frequency of the CTLA-4 LAG-3 T-cell subset in patients with newly diagnosed acute myeloid leukemia.新诊断急性髓系白血病患者中CTLA-4 LAG-3 T细胞亚群的频率更高。
Asia Pac J Clin Oncol. 2020 Apr;16(2):e12-e18. doi: 10.1111/ajco.13236. Epub 2019 Oct 15.
8
High expression of CD244 and SAP regulated CD8 T cell responses of patients with HTLV-I associated neurologic disease.高表达的 CD244 和 SAP 调节 HTLV-I 相关神经疾病患者的 CD8 T 细胞反应。
PLoS Pathog. 2009 Dec;5(12):e1000682. doi: 10.1371/journal.ppat.1000682. Epub 2009 Dec 4.
9
Fine specificity and molecular competition in SLAM family receptor signalling.SLAM 家族受体信号传导中的精细特异性和分子竞争。
PLoS One. 2014 Mar 18;9(3):e92184. doi: 10.1371/journal.pone.0092184. eCollection 2014.
10
Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4).SAP与EAT-2结合的白细胞细胞表面分子CD84、CD150（信号淋巴细胞激活分子）、CD229（Ly9）和CD244（2B4）的差异表达。
Tissue Antigens. 2004 Aug;64(2):132-44. doi: 10.1111/j.1399-0039.2004.00247.x.

引用本文的文献

1
Upregulation of CD244 promotes CD8 T cell exhaustion in patients with alveolar echinococcosis and a murine model.CD244 的上调促进了肺泡型包虫病患者和小鼠模型中 CD8 T 细胞衰竭。
Parasit Vectors. 2024 Nov 23;17(1):483. doi: 10.1186/s13071-024-06573-2.

本文引用的文献

1
CD244 represents a new therapeutic target in head and neck squamous cell carcinoma.CD244 是头颈部鳞状细胞癌的一个新的治疗靶点。
J Immunother Cancer. 2020 Mar;8(1). doi: 10.1136/jitc-2019-000245.
2
Immune Checkpoints in Circulating and Tumor-Infiltrating CD4 T Cell Subsets in Colorectal Cancer Patients.结直肠癌患者循环和肿瘤浸润 CD4 T 细胞亚群中的免疫检查点。
Front Immunol. 2019 Dec 17;10:2936. doi: 10.3389/fimmu.2019.02936. eCollection 2019.
3
Higher PD-1 expression concurrent with exhausted CD8+ T cells in patients with acute myeloid leukemia.
急性髓系白血病患者中，较高的PD-1表达与耗竭的CD8 + T细胞同时存在。
Chin J Cancer Res. 2017 Oct;29(5):463-470. doi: 10.21147/j.issn.1000-9604.2017.05.11.
4
Acute lymphoblastic leukemia: a comprehensive review and 2017 update.急性淋巴细胞白血病：全面综述及2017年更新
Blood Cancer J. 2017 Jun 30;7(6):e577. doi: 10.1038/bcj.2017.53.
5
T cells in multiple myeloma display features of exhaustion and senescence at the tumor site.多发性骨髓瘤中的T细胞在肿瘤部位表现出耗竭和衰老的特征。
J Hematol Oncol. 2016 Nov 3;9(1):116. doi: 10.1186/s13045-016-0345-3.
6
The 2016 revision of the World Health Organization classification of lymphoid neoplasms.《世界卫生组织淋巴组织肿瘤分类（2016年修订版）》
Blood. 2016 May 19;127(20):2375-90. doi: 10.1182/blood-2016-01-643569. Epub 2016 Mar 15.
7
Molecular and cellular insights into T cell exhaustion.对T细胞耗竭的分子和细胞层面的见解。
Nat Rev Immunol. 2015 Aug;15(8):486-99. doi: 10.1038/nri3862.
8
Long noncoding RNA derived from CD244 signaling epigenetically controls CD8+ T-cell immune responses in tuberculosis infection.源自CD244信号通路的长链非编码RNA通过表观遗传调控结核感染中CD8 + T细胞的免疫反应。
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3883-92. doi: 10.1073/pnas.1501662112. Epub 2015 Jul 6.
9
TIGIT and PD-1 impair tumor antigen-specific CD8⁺ T cells in melanoma patients.TIGIT和PD-1损害黑色素瘤患者体内肿瘤抗原特异性CD8⁺ T细胞。
J Clin Invest. 2015 May;125(5):2046-58. doi: 10.1172/JCI80445. Epub 2015 Apr 13.
10
PD-1 identifies the patient-specific CD8⁺ tumor-reactive repertoire infiltrating human tumors.PD-1 鉴定了浸润人类肿瘤的患者特异性 CD8⁺ 肿瘤反应性免疫组库。
J Clin Invest. 2014 May;124(5):2246-59. doi: 10.1172/JCI73639. Epub 2014 Mar 25.