评价急性白血病患者 CD8+ T 细胞中 CD244 及其衔接分子的 mRNA 表达。
Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8+ T Cells in Acute Leukemia.
机构信息
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Iran Biomed J. 2023 Jul 1;27(4):214-8. doi: 10.61186/ibj.3843.
BACKGROUND
This study investigated the role of the immune-checkpoint receptor (ICR), CD244, and its adapter molecules, in CD8+ T cells in acute leukemia.
METHODS
Blood samples were obtained from 21 acute lymphoblastic leukemia (ALL) and 6 acute myeloid leukemia (AML) patients and 20 control subjects. Relative gene expression of CD244, immune receptor tyrosine-based switch motif-associated protein (SA), EWS/FLI1-activated transcript 2 (EAT-2), and LncRNA-GSTT1-AS1 were evaluated using quantitative reverse transcription polymerase chain reaction.
RESULTS
Expression of CD244, SAP, and EAT-2 were significantly lower in CD8+ T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects.
CONCLUSION
The low SAP/CD244 expression ratio in CD8+ T cells in ALL suggests an inhibitory role for CD244 in ALL.
背景
本研究探讨了免疫检查点受体（ICR）CD244 及其衔接分子在急性白血病 CD8+T 细胞中的作用。
方法
采集 21 例急性淋巴细胞白血病（ALL）、6 例急性髓细胞白血病（AML）患者和 20 例对照者的血样。采用实时定量逆转录聚合酶链反应检测 CD244、免疫受体酪氨酸基开关基序相关蛋白（SAP）、EWS/FLI1 激活转录因子 2（EAT-2）和 LncRNA-GSTT1-AS1 的相对基因表达。
结果
与对照组相比，ALL 患者 CD8+T 细胞中 CD244、SAP 和 EAT-2 的表达明显降低。有趣的是，SAP 的表达明显低于 CD244，表明 SAP 与 CD244 的比值较低。此外，与对照组相比，AML 患者 SAP 的表达显著降低。与对照组相比，ALL 和 AML 患者 LncRNA-GSTT1-AS1 的表达无明显差异。
结论
ALL 患者 CD8+T 细胞中 SAP/CD244 表达比值较低，提示 CD244 在 ALL 中起抑制作用。
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