Wang Zi, Zhang Guangzhong, Zhang Haomin, Li Lingling
Beijing University of Chinese Medicine, Beijing 100029, China.
Department of Dermatology, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China.
Heliyon. 2023 Aug 11;9(8):e19109. doi: 10.1016/j.heliyon.2023.e19109. eCollection 2023 Aug.
Sphingosine-1-phosphate (S1P) is associated with the onset and severity of psoriasis, a chronic inflammatory skin disease linked to innate and adaptive immune responses. This study explores the therapeutic effect of Xiaoyin Jiedu Granules, a combination of traditional Chinese medicines, on psoriasis-like skin lesions in mice and the underlying mechanism. We used imiquimod (IMQ) to induce psoriasis-like dermatitis in mice; the effects of Xiaoyin Jiedu Granules on S1P receptors (S1PRs) were investigated using histology and immunohistochemistry. The effects of Xiaoyin Jiedu Granules on the proliferation, differentiation, and activation of the NF-κB pathway in keratinocytes were verified using quantitative polymerase chain reaction (qPCR) and western blotting analyses. CD4Th17 cells were screened using flow cytometry; the effects of Xiaoyin Jiedu Granules on the differentiation of Th17 cells and the content of related inflammatory factors were also verified. S1PR1-5 was highly expressed in psoriatic lesions. Xiaoyin Jiedu Granules significantly inhibited the secretion of proliferation-related proteins (K6, K16, K17, and IL-36γ) and proinflammatory cytokines (IL-17 and IL-22), transformation of Th17 cells, and activation of the NF-κB pathway and effectively alleviated IMQ-induced psoriasis-like dermatitis. Overall, our findings indicate that Xiaoyin Jiedu Granules have anti-inflammatory activity against S1PR expression, keratinocytes, and immune cells and can therefore mitigate psoriasis. Inhibiting the expression of S1PRs may be an effective treatment strategy against psoriasis.
鞘氨醇-1-磷酸（S1P）与银屑病的发病及严重程度相关，银屑病是一种与先天性和适应性免疫反应相关的慢性炎症性皮肤病。本研究探讨中药复方消银解毒颗粒对小鼠银屑病样皮肤损伤的治疗作用及其潜在机制。我们使用咪喹莫特（IMQ）诱导小鼠发生银屑病样皮炎；采用组织学和免疫组织化学方法研究消银解毒颗粒对S1P受体（S1PRs）的影响。运用定量聚合酶链反应（qPCR）和蛋白质免疫印迹分析验证消银解毒颗粒对角质形成细胞中NF-κB通路的增殖、分化及激活的影响。采用流式细胞术筛选CD4 Th17细胞；同时验证消银解毒颗粒对Th17细胞分化及相关炎症因子含量的影响。S1PR1-5在银屑病皮损中高表达。消银解毒颗粒显著抑制增殖相关蛋白（K6、K16、K17和IL-36γ）及促炎细胞因子（IL-17和IL-22）的分泌，抑制Th17细胞转化及NF-κB通路的激活，并有效减轻IMQ诱导的银屑病样皮炎。总体而言，我们的研究结果表明，消银解毒颗粒对S1PR表达、角质形成细胞及免疫细胞具有抗炎活性，因此可减轻银屑病症状。抑制S1PRs的表达可能是治疗银屑病的有效策略。
