Xiao Qiulin, Chen Bo, Zhu Zhenya, Yang Ting, Tao Enfu, Hu Chenmin, Zheng Wei, Tang Weihong, Shu Xiaoli, Jiang Mizu
Gastrointestinal Laboratory, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, Zhejiang, 310052, People's Republic of China.
Department of Gastroenterology, Hangzhou Children's Hospital, Hangzhou, Zhejiang, 310014, People's Republic of China.
Infect Drug Resist. 2023 Aug 21;16:5473-5483. doi: 10.2147/IDR.S410720. eCollection 2023.
To examine the association between the fecal microbiota of acute diarrhea in children and provide gut microbiota information related the acute diarrhea with rotavirus.
Children with acute diarrhea aged 3-60 months were selected for the study. Routine stool examination was performed, and stool samples were collected and stored at -80 °C until further analysis. Fecal microbial DNA was extracted, and DNA concentration and quality were detected. PCR amplification and 16S rDNA high-throughput sequencing analysis using the Illumina MiSeq platform were performed, and intestinal flora was statistically analyzed.
Children with acute diarrhea exhibited gut microbial dysbiosis. Lower microbial diversity and richness were observed in the viral enteritis and bacterial enteritis groups than in the control group. Composition of the microbiota in acute diarrhea differed from that in the control group. The / dramatically decreased in the viral enteritis and bacterial enteritis groups. However, the relative abundance of and increased, especially in the bacterial enteritis group. In addition, the relative abundance of had dramatically increased in the viral enteritis group. According to the Kyoto Encyclopedia of Genes and Genomes map analysis, the membrane transport dysfunction was caused by rotavirus infection, while the membrane transport dysfunction was more evident in bacterial infection.
Acute diarrhea infections cause fecal microbiota dysbiosis in children. Changes in fecal microflora in children suggest that the regulation of intestinal flora in children with acute diarrhea should be strengthened.
探讨儿童急性腹泻粪便微生物群之间的关联，并提供与轮状病毒引起的急性腹泻相关的肠道微生物群信息。
选取3至60个月的急性腹泻儿童进行研究。进行常规粪便检查，收集粪便样本并储存在-80°C直至进一步分析。提取粪便微生物DNA，检测DNA浓度和质量。使用Illumina MiSeq平台进行PCR扩增和16S rDNA高通量测序分析，并对肠道菌群进行统计分析。
急性腹泻儿童表现出肠道微生物群失调。病毒性肠炎组和细菌性肠炎组的微生物多样性和丰富度低于对照组。急性腹泻中微生物群的组成与对照组不同。在病毒性肠炎组和细菌性肠炎组中/显著下降。然而，和的相对丰度增加，尤其是在细菌性肠炎组。此外，病毒性肠炎组中的相对丰度显著增加。根据京都基因与基因组百科全书图谱分析，轮状病毒感染导致膜转运功能障碍，而膜转运功能障碍在细菌感染中更为明显。
急性腹泻感染导致儿童粪便微生物群失调。儿童粪便微生物群的变化表明，应加强对急性腹泻儿童肠道菌群的调节。
