von Wangenheim K H, Peterson H P, Feinendegen L E
Radiat Environ Biophys. 1986;25(2):93-106. doi: 10.1007/BF01211733.
Stem cells surviving radiation injury may carry defects which contribute to long-term effects. The ratio of 125-iododeoxyuridine (IUdR) uptake into spleens of lethally irradiated recipient mice between day 3 and day 5 after cell transfusion revealed reduced proliferative ability (PF) of spleen seeding cells in parallel with reduced CFU-S content of donors throughout the study period of one year after 5 Gy gamma irradiation. Additional data aided in evaluating possible mechanisms of PF reduction. Within the range of the graft sizes used, PF was independent of the numbers of cells or CFU-S transfused. Radiation-induced increase in loss of label between days 3 and 5 and prolonged doubling time of proliferating cells indicated enhancement of cell maturation and increase in mitotic cycle time. Increased IUdR uptake per transfused CFU-S suggested extra divisions of transit cells due to insufficiency in the stem cell compartment. It is concluded that persisting defects in surviving stem cells interfere in a complex way with cell proliferation in the hemopoietic system.
在辐射损伤中存活下来的干细胞可能带有导致长期影响的缺陷。在细胞输注后第3天至第5天之间，对接受致死剂量照射的受体小鼠脾脏摄取125 - 碘脱氧尿苷（IUdR）的情况进行检测，结果显示，在5 Gy伽马射线照射后的一年研究期内，脾脏接种细胞的增殖能力（PF）降低，同时供体的脾集落形成单位（CFU - S）含量也降低。其他数据有助于评估PF降低的可能机制。在所使用的移植细胞数量范围内，PF与输注的细胞数量或CFU - S数量无关。辐射导致第3天至第5天之间标记丢失增加以及增殖细胞的倍增时间延长，这表明细胞成熟增强且有丝分裂周期时间增加。每个输注的CFU - S摄取的IUdR增加表明，由于干细胞池不足，过渡细胞出现额外分裂。研究得出结论，存活干细胞中持续存在的缺陷以复杂的方式干扰造血系统中的细胞增殖。
