Basset Céline, Collin Laetitia, Vielh Philippe, Courtade-Saïdi Monique
Département d'anatomie et de cytologie pathologiques, institut universitaire du cancer Toulouse oncopôle, 1, avenue Irène Joliot Curie, 31059 Toulouse cedex, France.
Medipath Feuillants, 116, route d'Espagne, Hélios 3, 31100 Toulouse, France.
Ann Pathol. 2023 Nov;43(6):452-461. doi: 10.1016/j.annpat.2023.03.005. Epub 2023 Aug 26.
The International System for serous fluids cytopathology is a cytologic classification which purpose is to establish a consensus on diagnostic terminology. The exponential discovery of prognostic and theranostic molecular alterations in many cancers, particularly in advanced stages, led the authors to describe the indications and the feasibility of these new markers on cytological samples from serous effusions. The various immunocytochemistry techniques, FISH and those testing DNA and RNA are reported in regard to their ability to identify the main targets currently explored in routine practice. The vast majority of these crucial markers can be reliably tested on effusion fluids. The International System for serous effusion fluids also includes a chapter dedicated to peritoneal washings and the application of the classification to this particular type of sample. The objective is to "wash" the peritoneal cavity with a saline solution to unfix cells from the cavity's wall and collect those that have previously naturally detached. This procedure, performed before surgery of tumors and before any manipulation, allows a cytological analysis that specifies the staging of gynecological and non-gynecological tumors and excludes occult malignant cells in the presence of tumors appearing benign.
国际浆液性液体细胞病理学系统是一种细胞分类方法，其目的是在诊断术语上达成共识。在许多癌症中，尤其是晚期癌症中，预后和治疗诊断分子改变的快速发现，促使作者描述这些新标志物在浆液性积液细胞学样本中的应用指征和可行性。报告了各种免疫细胞化学技术、荧光原位杂交技术以及检测DNA和RNA的技术，涉及它们识别目前在常规实践中探索的主要靶点的能力。这些关键标志物中的绝大多数都可以在积液样本上进行可靠检测。国际浆液性积液系统还包括一章专门讨论腹膜冲洗以及该分类在这种特殊类型样本中的应用。目的是用盐溶液“冲洗”腹腔，以使细胞从腔壁上脱离并收集那些先前自然脱落的细胞。该程序在肿瘤手术前和任何操作前进行，可进行细胞学分析，明确妇科和非妇科肿瘤的分期，并在看似良性的肿瘤存在时排除隐匿性恶性细胞。
