Boyle D B, Coupar B E, Parsonson I M, Bagust T J, Both G W
Res Vet Sci. 1986 Jul;41(1):40-4.
Groups of cattle, sheep and poultry were inoculated with a recombinant vaccinia virus expressing the haemagglutinin of the swine influenza virus A/NJ/11/76. No adverse clinical responses were recorded and none of the animals developed a viraemia when inoculated with the recombinant or wild-type vaccinia virus. Recombinant virus reisolated from lesions in cattle was stable, maintaining its thymidine kinase negative phenotype and ability to express the swine influenza haemagglutinin. Antibodies to the influenza haemagglutinin were detected in cattle, sheep and poultry inoculated with the recombinant virus. While no animals inoculated with wild-type virus developed these antibodies, there was no detectable spread of either recombinant or wild-type virus from the inoculation sites or to in-contact uninoculated animals. The results indicate that recombinant vaccinia viruses can induce immune responses in cattle, sheep and poultry demonstrating their potential as vaccine vectors in a variety of important veterinary species.
将表达甲型猪流感病毒A/NJ/11/76血凝素的重组痘苗病毒接种到牛、羊和家禽群体中。未记录到不良临床反应，接种重组或野生型痘苗病毒后，动物均未出现病毒血症。从牛病变部位重新分离出的重组病毒稳定，保持其胸苷激酶阴性表型和表达猪流感血凝素的能力。在接种重组病毒的牛、羊和家禽中检测到针对流感血凝素的抗体。虽然接种野生型病毒的动物未产生这些抗体，但重组或野生型病毒均未从接种部位传播到接触的未接种动物。结果表明，重组痘苗病毒可在牛、羊和家禽中诱导免疫反应，证明其作为多种重要兽医物种疫苗载体的潜力。
