Wedman E E, Smith A W, Oliver R E
College of Veterinary Medicine, Oregon State University, Corvallis 97331-4802.
Am J Vet Res. 1988 Dec;49(12):2018-21.
Experiments concerned with the immunogenicity, pathogenicity, and transmissibility of a recombinant vaccinia:Sindbis virus were conducted. The WR strain of the recombinant vaccinia:Sindis virus was found to be infective for calves and mildly pathogenic, resulting in local tissue reaction. It was not transmissible to other calves. Also, it was found to be immunogenic when inoculated intradermally into calves, and antibody was produced against the parent vector virus (vaccinia) and the Sindbis antigen. Recombinant virus given IV to calves induced no detectable clinical signs, nor did the calves develop neutralizing antibodies. Furthermore, second-passage lesion material containing up to 10(7) tissue culture infective doses of the recombinant virus failed to induce development of lesions or illness in intradermally inoculated calves, and virus could not be recovered from the inoculation sites. In this series of experiments, this vaccinia recombinant given intradermally was immunogenic, mildly pathogenic at the local injection site only, and was not transmissible to contact animals, thus demonstrating the potential efficacy and safety of the WR strain of vaccinia virus when used as a live vector system in cattle.
开展了有关重组痘苗病毒-辛德毕斯病毒免疫原性、致病性和传播性的实验。发现重组痘苗病毒-辛德毕斯病毒的WR株对犊牛具有感染性且致病性轻微，会导致局部组织反应。它不会传播给其他犊牛。此外，当皮内接种到犊牛体内时，发现它具有免疫原性，会产生针对亲本载体病毒（痘苗病毒）和辛德毕斯抗原的抗体。静脉注射给犊牛的重组病毒未引发可检测到的临床症状，犊牛也未产生中和抗体。此外，含有高达10⁷组织培养感染剂量重组病毒的第二代病变材料未能在皮内接种的犊牛中引发病变或疾病，且无法从接种部位分离到病毒。在这一系列实验中，皮内接种的这种痘苗重组体具有免疫原性，仅在局部注射部位有轻微致病性，且不会传播给接触动物，从而证明了痘苗病毒WR株作为牛的活载体系统时的潜在有效性和安全性。
