Franke C A, Berry E S, Smith A W, Hruby D E
Res Vet Sci. 1985 Jul;39(1):113-5.
Genetic engineering techniques have been used to construct a vaccinia virus recombinant which contains and expresses togavirus (Sindbis) genetic information. Intradermal inoculation of this recombinant strain into calves caused a transient pock-type lesion at the site of inoculation and elicited the production of substantial levels of anti-Sindbis virus neutralising antibodies. These results suggest that recombinant vaccinia virus vaccines may have potential for use in veterinary medicine.
基因工程技术已被用于构建一种痘苗病毒重组体，该重组体包含并表达披膜病毒（辛德毕斯病毒）的遗传信息。将这种重组菌株皮内接种到小牛体内，在接种部位引起了短暂的痘疱型病变，并引发了大量抗辛德毕斯病毒中和抗体的产生。这些结果表明，重组痘苗病毒疫苗在兽医学中可能具有应用潜力。
