较高的白细胞介素-9水平与伴有代谢综合征的寻常型银屑病相关。

Higher IL-9 Level is Associated with Psoriasis Vulgaris Complicated by Metabolic Syndrome.

作者信息

Yan Liang, Yu Chongli, Zhao Zhenkai, Zhang Yuan, Wang Rui, Li Chengxin

机构信息

Department of Dermatology, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.

Department of Dermatology, General Hospital of Central Theater Command of PLA, Wuhan, Hubei, 430070, People's Republic of China.

出版信息

Clin Cosmet Investig Dermatol. 2023 Aug 23;16:2297-2307. doi: 10.2147/CCID.S422355. eCollection 2023.

DOI:10.2147/CCID.S422355

PMID:37641663

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10460594/

Abstract

PURPOSE

The underlying pathophysiology linking psoriasis vulgaris (PV) and metabolic syndrome (MetS) is not fully understood. The present study aimed to investigate the serum level of interleukin (IL)-9 and tissue levels of IL-9 and its receptor in PV patients with MetS and analyze the correlation of IL-9 levels with psoriasis disease severity and MetS.

METHODS

This study enrolled 75 PV patients with MetS, 57 PV patients without MetS, 20 healthy blood donors, and 7 healthy skin donors. Clinical, socio-demographic, and anthropometric data were obtained from all individuals. Fasting blood glucose, insulin, lipid profile levels, and serum levels of IL-9 and IL-17A were measured. The expression of IL-9 and its receptor in skin specimens in PV patients and healthy controls was determined using immunohistochemistry. Normal human epidermal keratinocytes were stimulated with five pro-inflammatory cytokines (tumor necrosis factor-α, oncostatin M, IL-22, IL-17A, and IL-1α) to establish a psoriatic keratinocyte model and subsequently treated with IL-9. Their mRNA levels of antimicrobial peptides and chemokines were measured using quantitative real-time polymerase chain reaction.

RESULTS

Serum level of IL-9 and tissue levels of IL-9 and its receptor were upregulated in PV patients with MetS. IL-9 level was positively correlated to IL-17A level; however, no significant correlation of IL-9 level with psoriasis area severity index was observed. IL-9 level had a positive correlation with the presence of MetS and its components. Correspondingly, IL-9 level positively correlated with waist circumference, body mass index, homeostasis model assessment-insulin resistance, blood pressure, and triglyceride level and negatively correlated with high-density lipoprotein cholesterol level. Additionally, IL-9 stimulated the expression of antimicrobial peptides and chemokines in a psoriatic keratinocyte model.

CONCLUSION

Our findings confirmed that higher IL-9 level is associated with PV complicated by MetS, suggesting that IL-9 may be a link between PV and MetS.

摘要

目的

寻常型银屑病（PV）与代谢综合征（MetS）之间潜在的病理生理联系尚未完全明确。本研究旨在调查合并MetS的PV患者的血清白细胞介素（IL）-9水平、IL-9及其受体的组织水平，并分析IL-9水平与银屑病疾病严重程度及MetS的相关性。

方法

本研究纳入了75例合并MetS的PV患者、57例未合并MetS的PV患者、20名健康献血者和7名健康皮肤供体。收集了所有个体的临床、社会人口学和人体测量数据。测量了空腹血糖、胰岛素、血脂水平以及IL-9和IL-17A的血清水平。采用免疫组织化学法测定PV患者和健康对照皮肤标本中IL-9及其受体的表达。用五种促炎细胞因子（肿瘤坏死因子-α、制瘤素M、IL-22、IL-17A和IL-1α）刺激正常人表皮角质形成细胞建立银屑病角质形成细胞模型，随后用IL-9处理。采用定量实时聚合酶链反应测定其抗菌肽和趋化因子的mRNA水平。

结果

合并MetS的PV患者血清IL-9水平以及IL-9及其受体的组织水平上调。IL-9水平与IL-17A水平呈正相关；然而，未观察到IL-9水平与银屑病面积严重程度指数有显著相关性。IL-9水平与MetS及其组分的存在呈正相关。相应地，IL-9水平与腰围、体重指数、稳态模型评估-胰岛素抵抗、血压和甘油三酯水平呈正相关，与高密度脂蛋白胆固醇水平呈负相关。此外，IL-9在银屑病角质形成细胞模型中刺激了抗菌肽和趋化因子的表达。