Department of Animal Sciences and D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville 32611-0910.
Departmento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais, BeloHorizonte, MG, Brazil CEP 31270-901.
J Dairy Sci. 2019 Jul;102(7):6587-6594. doi: 10.3168/jds.2019-16251. Epub 2019 May 15.
An inflammatory response is induced in the reproductive tract by deposition of semen during natural mating. This response might facilitate establishment and maintenance of pregnancy and alter the phenotype of the offspring by modifying the microenvironment of the reproductive tract. Here, we hypothesized that intrauterine infusion of 0.5 mL of seminal plasma at the time of artificial insemination (AI) in first-service lactating Holstein cows will improve pregnancy success after insemination. Cows were inseminated (511 primiparous cows inseminated with X-sorted semen, 554 multiparous cows inseminated with X-sorted semen, and 627 multiparous cows inseminated with conventional semen) using the Double-Ovsynch protocol. Cows were randomly assigned to receive intrauterine infusion of either 0.5 mL of seminal plasma or saline immediately after AI. There was no overall effect of seminal plasma infusion on the percentage of inseminated cows diagnosed pregnant at d 32 or 60 after AI, pregnancy loss, or percent of inseminated cows calving. If cows were inseminated with conventional semen, seminal plasma reduced pregnancies at d 32 and tended to reduce calvings. There was no effect of seminal plasma if cows were inseminated with X-sorted semen. Seminal plasma infusion increased the birth weight of heifer calves born using X-sorted semen but not conventional semen. These results do not support a beneficial effect of seminal plasma on pregnancy success after AI, but exposure to seminal plasma may program fetal development to affect phenotype at birth.
在自然交配过程中，精液的沉积会在生殖道中引发炎症反应。这种反应可能有助于妊娠的建立和维持，并通过改变生殖道的微环境来改变后代的表型。在这里，我们假设在第一次泌乳荷斯坦奶牛人工授精（AI）时，向子宫内输注 0.5 毫升精液会提高授精后的妊娠成功率。奶牛使用双-Ovsynch 方案进行授精（511 头初产奶牛用 X 分选精液授精，554 头经产奶牛用 X 分选精液授精，627 头经产奶牛用常规精液授精）。奶牛被随机分配接受子宫内输注 0.5 毫升精液或生理盐水，在 AI 后立即进行。精液输注对 AI 后 32 天或 60 天诊断为妊娠的授精奶牛的百分比、妊娠损失或授精奶牛的产犊百分比没有总体影响。如果奶牛用常规精液授精，精液会降低 32 天的妊娠率，并倾向于降低产犊率。如果用 X 分选精液授精，则精液无影响。精液输注增加了使用 X 分选精液的小母牛犊牛的出生体重，但对使用常规精液的小母牛犊牛没有影响。这些结果不支持精液对 AI 后妊娠成功的有益影响，但暴露于精液可能会影响胎儿发育，从而影响出生时的表型。
