Opt Lett. 2018 Nov 1;43(21):5190-5193. doi: 10.1364/OL.43.005190.
We developed a patterned-illumination second harmonic generation (PI-SHG) microscopy, which combines the principle of structured illumination reconstruction with SHG microscopy for label-free super-resolution imaging. We confirmed that PI-SHG microscopy can achieve 1.59-time resolution improvement compared to conventional SHG microscopy by imaging nanowire samples. We further demonstrated three-dimensional PI-SHG microscopy in imaging ex vivo collagen fibrils in rat scleras.
我们开发了一种基于图案化照明的二次谐波产生（PI-SHG）显微镜，它结合了结构光照明显微术和 SHG 显微镜的原理，用于无标记的超分辨率成像。我们通过对纳米线样品进行成像，证实了 PI-SHG 显微镜可以实现比传统 SHG 显微镜高 1.59 倍的分辨率提升。我们进一步在大鼠巩膜的胶原纤维的三维成像中演示了三维 PI-SHG 显微镜。
