PCCM, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, China.
Can Respir J. 2023 Aug 21;2023:2983092. doi: 10.1155/2023/2983092. eCollection 2023.
Inhalation of nebulized TP has received little attention in the past. Here, we intend to investigate the effect of nebulized inhaled TP on airway inflammation in a mouse model of asthma. 29 SPF BALB/c mice were divided into four groups: blank control (Blk, = 5), normal saline (NS, = 8), dexamethasone (Dex, = 8), and TP ( = 8). During the process of sensitization, mice in the three intervention groups were treated with nebulized NS, an injection of Dex, and nebulized triptolide, respectively. Then bronchoalveolar lavage fluid (BALF), peripheral blood, and lung tissue were collected. Relevant cytokines, transcriptional factors, and CD4+Th17+ T cell proportions were assessed and compared. IL-6, IL-17, IL-23, and TGF-1 demonstrated a significant difference between groups in the following order: Dex < TP < NS ( ≤ 0.001), while IL-10 changed in the opposite direction ( < 0.001). At the transcriptional level in lung tissue, the Ct value of IL-17 in the Dex group was significantly higher than in the NS and TP groups ( < 0.001). Meanwhile, it was higher in the TP group than in the NS group ( < 0.001). The Ct value of RORt demonstrated a significant difference among three groups in the following order: Dex > TP > NS ( < 0.001). An opposite trend of FoxP3 Ct value was revealed in the order: NS > TP > Dex. The proportion of CD4+Th17+ cells was 9.53 ± 2.74% in the NS group, 4.23 ± 2.26% in the Dex group, and 6.76 ± 2.99% in the TP group, which shows significant differences between the NS and Dex ( < 0.001) or NS and TP groups ( < 0.05). Inhalation of nebulized triptolide can play a role in suppressing airway inflammation with inflammatory cytokines and transcriptional factors reduced and CD4+Th17+ T cells dampened, also in a manner less than injected dexamethasone.
雾化吸入雷公藤红素（Triptolide，TP）在过去受到的关注较少。本研究旨在探讨雾化吸入 TP 对哮喘小鼠模型气道炎症的影响。29 只 SPF BALB/c 小鼠分为 4 组：空白对照组（Blk，n=5）、生理盐水组（NS，n=8）、地塞米松组（Dex，n=8）和 TP 组（n=8）。在致敏过程中，3 个干预组分别用雾化 NS、地塞米松注射和雾化雷公藤红素处理。然后收集支气管肺泡灌洗液（BALF）、外周血和肺组织。评估并比较相关细胞因子、转录因子和 CD4+Th17+T 细胞比例。IL-6、IL-17、IL-23 和 TGF-β1 组间差异有统计学意义，按 Dex<TP<NS（≤0.001）的顺序排列，而 IL-10 则呈相反方向变化（<0.001）。在肺组织的转录水平上，Dex 组 IL-17 的 Ct 值明显高于 NS 和 TP 组（<0.001），TP 组又高于 NS 组（<0.001）。RORt 的 Ct 值在 3 组间差异有统计学意义，按 Dex>TP>NS（<0.001）的顺序排列。FoxP3 Ct 值呈相反趋势，按 NS>TP>Dex 的顺序排列。NS 组 CD4+Th17+细胞比例为 9.53%±2.74%，Dex 组为 4.23%±2.26%，TP 组为 6.76%±2.99%，NS 组与 Dex 组（<0.001）或 NS 组与 TP 组（<0.05）比较差异有统计学意义。雾化吸入雷公藤红素可通过降低炎症细胞因子和转录因子，减少 CD4+Th17+T 细胞，发挥抑制气道炎症的作用，且作用弱于注射用地塞米松。
