Jeddi Mohamed, El Hachlafi Naoufal, Fadil Mouhcine, Benkhaira Nesrine, Jeddi Samir, Benziane Ouaritini Zineb, Fikri-Benbrahim Kawtar
Laboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Imouzzer Road, Fez, Morocco.
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30 000, Morocco.
Adv Pharmacol Pharm Sci. 2023 Aug 21;2023:5593350. doi: 10.1155/2023/5593350. eCollection 2023.
This study aims to identify the volatile profile of three essential oils obtained from (EPEO), (OMEO), and (LBEO) and to examine their combined antibacterial activity that affords the optimal inhibitory ability against and using simplex-centroid mixture design and checkerboard assay. Essential oils (EOs) were isolated by hydrodistillation and characterized using gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupled with flame-ionization detector (GC-FID). The antibacterial activity was performed using disc diffusion and microdilution assays. The chemical analysis revealed that 1,8-cineole (23.75%), p-cymene (22.47%), and -pinene (11.20%) and p-menthane-1,8-diol (18.19%), -pinene (10.81%), and D-germacrene (9.17%) were the main components detected in and EOs, respectively. However, EO was mainly represented by linalool (24.40%) and linalyl acetate (18.68%). The EPEO, LBEO, and OMEO had a strong antibacterial effect on with minimal inhibitory concentrations (MICs) values ranging from 0.25 to 0.5% (v/v). Furthermore, the combination of 1/2048 MIC + 1/4 MIC showed a synergistic antibacterial effect on with a FIC index of 0.25, while the formulation of 1/4 MIC + 1/4 MIC demonstrated an antibacterial synergistic activity on with a FIC index of 0.5. Moreover, the simplex-centroid mixture design reported that the most effective combinations on and correspond to 32%/28%/40% and 35%/30%/35% of , and respectively. Presented information highlights the action of antibacterial formulations of these EOs and suggests their potential applications as alternatives to commercialized drugs to contract the development of bacteria causing serious infections and food deterioration.
本研究旨在鉴定从[具体植物1]（EPEO）、[具体植物2]（OMEO）和[具体植物3]（LBEO）中获得的三种精油的挥发性成分，并使用单纯形重心混合设计和棋盘法检测它们的联合抗菌活性，以获得对[目标细菌1]和[目标细菌2]的最佳抑制能力。通过水蒸馏法分离精油（EOs），并使用气相色谱 - 质谱联用仪（GC - MS）和气相色谱 - 火焰离子化检测器（GC - FID）对其进行表征。使用纸片扩散法和微量稀释法进行抗菌活性检测。化学分析表明，1,8 - 桉叶素（23.75%）、对伞花烃（22.47%）和α - 蒎烯（11.20%）以及对薄荷烷 - 1,8 - 二醇（18.19%）、α - 蒎烯（10.81%）和D - 吉马烯（9.17%）分别是在EPEO和OMEO精油中检测到的主要成分。然而，LBEO精油主要由芳樟醇（24.40%）和乙酸芳樟酯（18.68%）组成。EPEO、LBEO和OMEO对[目标细菌1]具有较强的抗菌作用，最低抑菌浓度（MICs）值范围为0.25%至0.5%（v/v）。此外，1/2048 MIC + 1/4 MIC的组合对[目标细菌1]显示出协同抗菌作用，FIC指数为0.25，而1/4 MIC + 1/4 MIC的配方对[目标细菌2]表现出抗菌协同活性，FIC指数为0.5。此外，单纯形重心混合设计表明，对[目标细菌1]和[目标细菌2]最有效的组合分别对应于EPEO、LBEO和OMEO的32%/28%/40%和35%/30%/35%。所呈现的信息突出了这些精油抗菌配方的作用，并表明它们作为商业化药物的替代品的潜在应用，以对抗导致严重感染和食品变质的细菌的发展。
