Laboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Physico-Chemical Laboratory of Inorganic and Organic Materials, Materials Science Center (MSC), Ecole Normale Supérieure, Mohammed V University in Rabat, Rabat, Morocco.
Exp Parasitol. 2023 Apr;247:108472. doi: 10.1016/j.exppara.2023.108472. Epub 2023 Jan 21.
Essential Oils (EOs) known since Antiquity, and initially obtained by maceration of aromatic plants, are used as diseases' remedies because they contain valuable therapeutic components. Antimicrobial effect's evaluation of formulations established by an augmented centered mixing plan for three Moroccan medicinal and aromatic plants' essential oils (EOs): Thymus satureioides (T. satureioides), Myrtus communis (M. communis) and Artemisia herba alba (A. herba alba) against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida tropicalis (C. tropicalis). The identification of E.Os chemical compounds was made using Gaz chromatography (GC) and Gaz chromatography coupled to Mass spectrometry (GC/MS).Whereas, the optimization of their antimicrobial effect was performed by experimental design methodology (mixture design) coupled to microdilution method based on Minimal Inhibitory Concentrations (MICs) determination. Results showed that the main components are borneol (34.26%) and α-pinene (31.21%); borneol (27.15%) and 1,8-cineole (21.33%); camphor (14.67%), chrysanthenone (14.10%), and β-thujone (12.60%) for T. satureioides, M. communis and A. herba alba respectively. Optimal mixtures composed of 60% and 40% of T. satureioides and M. communis E.Os; 72% and 28% of T. satureioides and A. Herba alba E.Os; 75% of thyme E.O and 25% of mugwort E.O; predicted the highest antimicrobial effect against E. coli, S. aureus and C. tropicalis, respectively. This study highlights the antimicrobial effect of EOs tertiary mixtures through using mixture design methodology, which may contribute to a successful application in pharmaceutical, food, or cosmetic industries.
精油（EOs）自古以来就为人所知，最初是通过浸渍芳香植物获得的，因其含有有价值的治疗成分而被用作疾病的治疗方法。采用中心复合设计法建立了三种摩洛哥药用和芳香植物精油（EOs）的配方，评估其对大肠杆菌（E. coli）、金黄色葡萄球菌（S. aureus）和热带假丝酵母（C. tropicalis）的抗菌效果。EOs 化学成分的鉴定采用气相色谱（GC）和气相色谱-质谱联用（GC/MS）法。同时，通过实验设计方法（混合设计）结合基于最小抑菌浓度（MICs）测定的微量稀释法对其抗菌效果进行优化。结果表明，主要成分是龙脑（34.26%）和α-蒎烯（31.21%）；龙脑（27.15%）和 1,8-桉叶素（21.33%）；樟脑（14.67%）、菊花酮（14.10%）和β-侧柏酮（12.60%），分别为 T. satureioides、M. communis 和 A. herba alba。由 T. satureioides 和 M. communis EOs 组成的 60%和 40%的最佳混合物；T. satureioides 和 A. Herba alba EOs 的 72%和 28%；百里香 E.O 的 75%和艾蒿 E.O 的 25%，分别预测对大肠杆菌、金黄色葡萄球菌和热带假丝酵母具有最高的抗菌效果。本研究通过使用混合设计方法突出了 EOs 三元混合物的抗菌效果，这可能有助于在制药、食品或化妆品行业成功应用。
