Department of Health Management, The People's Hospital of Guangxi Zhuang Autonomous Region and Research Center of Health Management, Guangxi Academy of Medical Sciences, Nanning, China.
Department of Oncology, Guangxi Medical University Cancer Hospital, Nanning, China.
Front Immunol. 2023 Aug 14;14:1202150. doi: 10.3389/fimmu.2023.1202150. eCollection 2023.
Interferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in specific tumors or diseases, but the relevant role of IFN-γ in pan-cancer remains uncertain.
TCGA and GTEx RNA expression data and clinical data were downloaded. Additionally, we analyzed the role of IFN-γ on tumors by using a bioinformatic approach, which included the analysis of the correlation between IFN-γ in different tumors and expression, prognosis, functional status, TMB, MSI, immune cell infiltration, and TIDE. We also developed a PPI network for topological analysis of the network, identifying hub genes as those having a degree greater than IFN-γ levels.
IFN-γ was differentially expressed and predicted different survival statuses in a majority of tumor types in TCGA. Additionally, IFN-γ expression was strongly linked to factors like infiltration of T cells, immune checkpoints, immune-activating genes, immunosuppressive genes, chemokines, and chemokine receptors, as well as tumor purity, functional statuses, and prognostic value. Also, prognosis, CNV, and treatment response were all substantially correlated with IFN-γ-related gene expression. Particularly, the IFN-γ-related gene STAT1 exhibited the greatest percentage of SNVs and the largest percentage of SNPs in UCEC. Elevated expression levels of IFN-γ-related genes were found in a wide variety of tumor types, and this was shown to be positively linked to drug sensitivity for 20 different types of drugs.
IFN-γ is a good indicator of response to tumor immunotherapy and is likely to limit tumor progression, offering a novel approach for immunotherapy's future development.
干扰素-γ（IFN-γ），通常称为 II 型干扰素，是一种关键的细胞因子，协调肿瘤免疫过程，在肿瘤免疫治疗研究中受到广泛关注。以前的研究已经讨论了 IFN-γ在特定肿瘤或疾病中的作用和机制，但 IFN-γ在泛癌中的相关作用仍不确定。
下载 TCGA 和 GTEx RNA 表达数据和临床数据。此外，我们还通过生物信息学方法分析了 IFN-γ在肿瘤中的作用，包括分析不同肿瘤中 IFN-γ与表达、预后、功能状态、TMB、MSI、免疫细胞浸润和 TIDE 的相关性。我们还开发了一个 PPI 网络，用于对网络进行拓扑分析，确定具有大于 IFN-γ水平的度的枢纽基因。
IFN-γ在 TCGA 中的大多数肿瘤类型中差异表达，并预测了不同的生存状态。此外，IFN-γ表达与 T 细胞浸润、免疫检查点、免疫激活基因、免疫抑制基因、趋化因子和趋化因子受体等因素密切相关，与肿瘤纯度、功能状态和预后价值密切相关。此外，预后、CNV 和治疗反应与 IFN-γ相关基因表达均显著相关。特别是，IFN-γ相关基因 STAT1 在 UCEC 中具有最大的 SNV 比例和最大的 SNP 比例。在多种肿瘤类型中发现 IFN-γ相关基因表达水平升高，并且与 20 种不同类型药物的药物敏感性呈正相关。
IFN-γ是肿瘤免疫治疗反应的良好指标，可能限制肿瘤进展，为免疫治疗的未来发展提供新途径。
