Department of Pathobiology, Auburn University, College of Veterinary Medicine, Auburn, AL, USA.
Research Animal Resources and Compliance, University of Wisconsin-Madison, Madison, WI, USA.
J Vet Diagn Invest. 2023 Nov;35(6):698-703. doi: 10.1177/10406387231193965. Epub 2023 Aug 30.
A 4-y-old female and 3-y-old male rhesus macaque (), both housed in the same facility, died unexpectedly within 2 wk. Postmortem examination revealed severe gastric dilation in both macaques and gastric emphysema in the female macaque. Histologically, bacteria consistent with sp. were present in both macaques within the lungs and lumen of the trachea, esophagus, and gastrointestinal (GI) tract without associated inflammation. Additionally, in the female macaque, the bacteria were found in the gastric mucosa and associated with emphysematous spaces in the gastric wall without associated inflammation. PCR and Sanger sequencing of amplicons were subsequently performed on GI contents and non-alimentary tissues from the 2 affected monkeys and on comparative samples from unaffected rhesus monkeys in the same facility and an adjacent primate facility. The cases were compared using the 2-tailed Fisher exact test (-value at 95% confidence). PCR identified in GI contents of both affected and unaffected monkeys ( = 0.6084) and in non-alimentary tissues of affected monkeys only ( = 0.0083). These results suggest that the presence of sp. in non-alimentary tissues is associated with gastric distension, gas accumulation, and unexpected death in nonhuman primates.
一雌一雄两只 4 岁和 3 岁的恒河猴（），被关在同一个设施里，在两周内意外死亡。尸检显示，两只猕猴都有严重的胃扩张和雌性猕猴的胃积气。组织学检查发现，两只猕猴的肺部以及气管、食管和胃肠道（GI）腔内都存在与 sp. 一致的细菌，但没有伴随炎症。此外，在雌性猕猴的胃黏膜和胃壁的积气腔中也发现了这种细菌，没有伴随炎症。随后对来自 2 只受影响猕猴的 GI 内容物和非食用组织以及同一设施和相邻灵长类动物设施中未受影响的恒河猴的对照样本进行了 PCR 和 Sanger 测序。使用双侧 Fisher 精确检验（95%置信区间的 值）对病例进行比较。PCR 鉴定出 GI 内容物中存在 ，受影响和未受影响的猴子都有（=0.6084），仅受影响的猴子的非食用组织中存在（=0.0083）。这些结果表明，sp. 存在于非食用组织中与非人类灵长类动物的胃扩张、气体积聚和意外死亡有关。
