Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776, Warsaw, Poland.
Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776, Warsaw, Poland.
Appl Biochem Biotechnol. 2024 Jun;196(6):3274-3316. doi: 10.1007/s12010-023-04705-5. Epub 2023 Aug 30.
Due to the growing demand for natural carotenoids, researchers have been searching for strains that are capable of efficient synthesis of these compounds. This study tested 114 red yeast strains collected from various natural environments and food specimens in Poland. The strains were isolated by their ability to produce red or yellow pigments in rich nutrient media. According to potential industrial significance of the carotenoids, both their total production and share of individual carotenoids (β-carotene, γ-carotene, torulene, and torularhodin) were analyzed. The total content of carotenoid pigments in the yeast dry matter ranged from 13.88 to 406.50 µg/g, and the percentages of individual carotenoids highly varied among the strains. Most of the yeast isolates synthesized torulene at the highest amount. Among the studied strains, isolates with a total carotenoid content in biomass greater than 200 µg/g and those containing more than 60% torularhodin were selected for identification (48 strains). The identified strains belonged to six genera: Rhodotorula, Sporidiobolus, Sporobolomyces, Buckleyzyma, Cystofilobasidium, and Erythrobasidium. The largest number of isolates belonged to Rhodotorula babjevae (18), Rhodotorula mucilaginosa (7), Sporidiobolus pararoseus (4), and Rhodotorula glutinis (4).
由于对天然类胡萝卜素的需求不断增长，研究人员一直在寻找能够高效合成这些化合物的菌株。本研究测试了从波兰各种自然环境和食品样本中收集的 114 株红色酵母菌株。这些菌株通过在丰富的营养培养基中产生红色或黄色色素的能力被分离出来。根据类胡萝卜素的潜在工业意义，分析了它们的总产量和单个类胡萝卜素（β-胡萝卜素、γ-胡萝卜素、番茄红素和番茄红素）的比例。酵母干物质中类胡萝卜素色素的总量范围为 13.88 至 406.50μg/g，不同菌株之间类胡萝卜素的比例差异很大。大多数酵母分离物以最高量合成番茄红素。在所研究的菌株中，选择总生物量中类胡萝卜素含量大于 200μg/g 且含有超过 60%番茄红素的菌株进行鉴定（48 株）。鉴定出的菌株属于六个属：红酵母属、节孢子菌属、节孢酵母属、巴克氏酵母属、胶孢球腔菌属和红酵母属。分离株数量最多的是 babjevae 红酵母（18 株）、粘红酵母（7 株）、pararoseus 节孢子菌（4 株）和glutinis 红酵母（4 株）。
