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从中国一个油田中分离到的一个新型产生物表面活性剂的菌株,ME46。

A new biosurfactant-producing strain, ME46, isolated from an oil field in China.

机构信息

State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.

Engineering Research Center of Microbial Enhanced Oil Recovery (MEOR), East China University of Science and Technology, Shanghai, People's Republic of China.

出版信息

Environ Technol. 2024 Aug;45(20):4089-4095. doi: 10.1080/09593330.2023.2240947. Epub 2023 Aug 30.

Abstract

The genus contains twelve species significant in the synthesis of cellulose-degrading enzymes and phenylalanine dehydrogenase, isolated mainly from marine sedimentary environments. Here, we report a new biosurfactant-producing strain, ME46, isolated from Daqing oil field in China. The biosurfactant extracted from Strain ME46 was determined as surfactin, one of the representative families of lipopeptide biosurfactants. The yield of the surfactin produced by strain ME46 was 0.62 g·L as determined by high-performance liquid chromatography, and the critical micelle concentration (CMC) of the surfactin was estimated to be about 68 mg·L and the surface tension at CMC was 35.1 mN·m. This study extended our knowledge about the role of the species in the ecosystem of natural environments such as the oil field.

摘要

该属包含 12 个种,主要从海洋沉积物环境中分离得到,在纤维素降解酶和苯丙氨酸脱氢酶的合成中具有重要作用。在这里,我们报告了一种新的生物表面活性剂产生菌株,即来自中国大庆油田的 ME46。从菌株 ME46 中提取的生物表面活性剂被确定为表面活性剂,它是脂肽生物表面活性剂的代表性家族之一。通过高效液相色谱法测定,菌株 ME46 产生的表面活性剂产量为 0.62g·L,表面活性剂的临界胶束浓度(CMC)估计约为 68mg·L,CMC 时的表面张力为 35.1mN·m。本研究扩展了我们对该物种在自然环境(如油田)生态系统中作用的认识。

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