Guangxi Medical University, Nanning, China.
Department of Respiratory Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
Immun Inflamm Dis. 2023 Aug;11(8):e916. doi: 10.1002/iid3.916.
A systemic and local inflammatory immune imbalance is thought to be the cause of traumatic tracheal stenosis (TS). However, with CD4 T lymphocytes being the predominant immune cells in TS, the mechanism of action and recruitment has not been described. In our research, using flow cytometry, ELISA, immunofluorescence, and Transwell chamber assays, the expression, distribution, and potential chemotactic function of CD4 T cells in TS patients were examined before and after treatment. The results showed that the untreated group had significantly more CD4 T cells and their secreted TGF-β1 than the treated group. Additionally, the untreated group's CD4 T cells showed a significant rise in CCL22 and CCL1, as well as a larger proportion of CCR4 and CCR8. CD4 T cells and CD68 macrophages located in TS also expressed CCL1 and CCL22. In vitro, anti-CCL1 and anti-CCL22 can partially block the chemoattractant effect of TS bronchoalveolar lavage (BAL) on purified CD4 T cells. The findings of this study indicated that TS contained unbalanced CD4 immune cells that were actively recruited locally by CCR4/CCL22 and CCR8/CCL1. As a result, it is anticipated that CD4 immune rebalancing can serve as a novel treatment for TS.
全身性和局部炎症免疫失衡被认为是创伤性气管狭窄（TS）的原因。然而，由于 CD4 T 淋巴细胞是 TS 中的主要免疫细胞，其作用机制和募集过程尚未描述。在我们的研究中，使用流式细胞术、ELISA、免疫荧光和 Transwell 室分析，在治疗前后检查了 TS 患者 CD4 T 细胞的表达、分布和潜在趋化功能。结果表明，未经治疗组的 CD4 T 细胞及其分泌的 TGF-β1 明显多于治疗组。此外，未经治疗组的 CD4 T 细胞 CCL22 和 CCL1 明显升高，CCR4 和 CCR8 的比例也较大。位于 TS 的 CD4 T 细胞和 CD68 巨噬细胞也表达 CCL1 和 CCL22。在体外，抗 CCL1 和抗 CCL22 可部分阻断 TS 支气管肺泡灌洗液（BAL）对纯化的 CD4 T 细胞的趋化作用。这项研究的结果表明，TS 含有不平衡的 CD4 免疫细胞，这些细胞通过 CCR4/CCL22 和 CCR8/CCL1 被局部主动募集。因此，预计 CD4 免疫平衡可以作为 TS 的一种新的治疗方法。
