Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
Department of Conservative Dentistry, College of Dentistry, Gangnam Severance Hospital, Yonsei University, Seoul, Republic of Korea.
Sci Rep. 2023 Aug 30;13(1):14243. doi: 10.1038/s41598-023-30351-2.
The aim of this study is to evaluate intratubular crystal formation from the experimental material consisting of dicalcium silicate (CS) and tricalcium silicate (CS) with nano-scaled particle size. A total of twenty-four specimens were made by isolating 8 mm of the cervical part centered at the cementoenamel junction of extracted premolars. Twelve specimens were not treated and considered as control. The experimental material was applied to the other twelve specimens by brushing for 10,000 strokes. Each group was randomly divided into four subgroups according to the period of immersion in phosphate buffer saline (PBS) for 1, 30, 60, and 90 days each. The specimens were sectioned longitudinally and examined with scanning electron microscopy and energy dispersion X-ray spectroscopy. The intratubular crystal were formed in PBS and densely filled the dentinal tubules over time. The crystal formation occurred at a depth of more than 50 μm from the dentin surface. The Ca/P ratio of formed intratubular crystals was 1.68 after 3 months. The experimental material consisting of CS and CS with a nanoscale particle size can form hydroxyapatite-like crystals in dentinal tubules in PBS, and there is a possibility of reducing dentin hypersensitivity by blocking the dentinal fluid flow.
本研究旨在评估由纳米级粒径的硅酸二钙（CS）和硅酸三钙（CS）组成的实验材料在管内的晶体形成情况。从 24 颗提取前磨牙的牙颈部釉牙骨质界中心处截取 8mm 牙体，共制作 24 个样本。其中 12 个样本未进行任何处理，作为对照组。通过刷涂，将实验材料施加于其余 12 个样本上，共 10000 次。每个组根据在磷酸盐缓冲盐水（PBS）中浸泡的时间随机分为 4 个亚组，每组浸泡时间分别为 1、30、60 和 90 天。将样本纵向切割，使用扫描电子显微镜和能量色散 X 射线能谱仪进行检查。在 PBS 中形成了管内晶体，随着时间的推移，晶体逐渐填满牙本质小管。晶体形成于距牙本质表面 50μm 以上的深度。在 3 个月后，形成的管内晶体的 Ca/P 比为 1.68。由 CS 和 CS 组成的纳米级粒径的实验材料可在 PBS 中的牙本质小管内形成类羟基磷灰石晶体，通过阻塞牙本质液流，有降低牙本质敏感性的可能性。
