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硅酸三钙诱导矿化封闭牙本质小管。

Tricalcium silicate induced mineralization for occlusion of dentinal tubules.

机构信息

Shanghai Institute of Ceramics, Chinese Academy of Sciences, China.

出版信息

Aust Dent J. 2011 Jun;56(2):175-80. doi: 10.1111/j.1834-7819.2011.01321.x.

Abstract

BACKGROUND

The aim of this study was to evaluate the dentinal tubule occluding ability of tricalcium silicate (Ca(3) SiO(5) or C(3) S) in vitro.

METHODS

Dentine discs were prepared from extracted caries-free human third molars and etched with 0.02 M citric acid (pH 4) for 3 minutes in order to produce patent dentinal tubules. Tricalcium silicate pastes of different viscosities were applied to the surface of the dentine specimens for 3 minutes and then removed with a swab followed by deionized water rinse. The dentine specimens were immersed in artificial saliva for 7 days. The tubule occlusion, sealing depth and chemical composition of the dentine specimens and bonding force between the dentine matrix and mineralized layer were evaluated by scanning electron microscopy, energy dispersion X-ray spectroscopy, X-ray diffraction analysis and a nanoscratch test.

RESULTS

A homogeneous layer of mineral crystals was precipitated onto the dentine surface and caused significant occlusion of the dentinal tubules. The dentine permeability and sealing depth of the C(3) S increased with the decrease of viscosity of the paste. With a proper viscosity of C(3) S pastes, the interface between the mineralized layer and the dentine matrix bonded well.

CONCLUSIONS

This study suggests that C(3) S may be an effective agent for the treatment of dentine hypersensitivity.

摘要

背景

本研究旨在评估硅酸三钙(Ca(3)SiO(5)或 C(3)S)在体外封闭牙本质小管的能力。

方法

从无龋的人第三磨牙中制备牙本质圆盘,并使用 0.02 M 柠檬酸(pH 4)蚀刻 3 分钟,以产生明显的牙本质小管。将不同粘度的硅酸三钙糊剂涂在牙本质标本表面 3 分钟,然后用棉签去除,再用去离子水冲洗。将牙本质标本浸泡在人工唾液中 7 天。通过扫描电子显微镜、能量色散 X 射线能谱、X 射线衍射分析和纳米划痕试验评估牙本质标本的小管封闭、密封深度和化学组成以及牙本质基质与矿化层之间的粘结力。

结果

在牙本质表面沉淀出一层均匀的矿物质晶体,导致牙本质小管显著封闭。C(3)S 的牙本质渗透性和密封深度随糊剂粘度的降低而增加。适当粘度的 C(3)S 糊剂可使矿化层与牙本质基质之间的界面结合良好。

结论

本研究表明,C(3)S 可能是治疗牙本质过敏的有效药物。

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