Gu Jia, Qin Gang, Jiang Liang, Xu Wei, Wang Yuanyuan, Liao Jiangxue, Pan Hongzhu, Liang Zhuoping
Department of Otolaryngology Head and Neck Surgery, The Affiliated Hospital of Southwest Medical University, Taiping Street & NO. 25, Luzhou, 646000, Sichuan, China.
Allergy Asthma Clin Immunol. 2023 Aug 30;19(1):79. doi: 10.1186/s13223-023-00834-y.
Interleukin (IL)-36 family cytokines have received increasing attention, especially in the fields of inflammation and immunity research. However, whether IL-36 family cytokine levels are correlated with the results of the assessment of allergic rhinitis (AR) and affect the severity of AR remains unknown. Therefore, this study aimed to investigate the correlations between IL-36 family cytokine levels and subjective and objective assessment results and to further analyze the possible mechanisms of IL-36 family cytokines in the development of AR.
An enzyme-linked immunosorbent assay (ELISA) was used to detect the concentrations of the IL-36 family cytokines IL-36α, IL-36β, IL-36γ, IL-36Ra, and IL-38 in the peripheral blood of patients with AR. The condition of patients with AR was assessed by 22-item sino-nasal outcome test (SNOT-22) score, visual analogue scale (VAS) scores for disease severity, and serum inhalant allergen immunoglobulin E (IgE) detection. Correlations between IL-36 family cytokine levels and subjective and objective assessment results in patients with AR were analyzed.
The concentration of IL-36α in the peripheral blood of patients with AR was the highest, and the concentration of IL-36β was the lowest. The concentration of IL-36α was higher in juvenile patients than in adult patients, and there was a difference in the IL-36Ra level between the perennial allergen group and the seasonal allergen group. There was a positive correlation between IL-36α level and IL-36γ level, IL-36γ level and IL-36Ra level, and IL-36Ra level and IL-38 level, and IL-36β level was positively correlated with IL-36Ra and IL-38 levels, respectively. IL-36α level was positively correlated with VAS score for nasal congestion symptom. IL-36β level was positively correlated with the total VAS score for ocular symptoms and VAS scores for ocular itching and eye pain symptoms. However, there was no correlation between the levels of all cytokines in IL-36 family and SNOT-22 score, the number of positive inhaled allergens, or the highest positive intensity of allergen specific immunoglobulin E (sIgE).
Peripheral blood IL-36 family cytokines play an important role in AR, and the concentrations of IL-36α and IL-36β were related to the severity of symptoms in patients with AR.
白细胞介素（IL）-36家族细胞因子受到越来越多的关注，尤其是在炎症和免疫研究领域。然而，IL-36家族细胞因子水平是否与过敏性鼻炎（AR）的评估结果相关以及是否影响AR的严重程度仍不清楚。因此，本研究旨在探讨IL-36家族细胞因子水平与主观和客观评估结果之间的相关性，并进一步分析IL-36家族细胞因子在AR发病中的可能机制。
采用酶联免疫吸附测定（ELISA）法检测AR患者外周血中IL-36家族细胞因子IL-36α、IL-36β、IL-36γ、IL-36Ra和IL-38的浓度。通过22项鼻鼻窦结局测试（SNOT-22）评分、疾病严重程度视觉模拟量表（VAS）评分以及血清吸入性变应原免疫球蛋白E（IgE）检测对AR患者的病情进行评估。分析AR患者IL-36家族细胞因子水平与主观和客观评估结果之间的相关性。
AR患者外周血中IL-36α浓度最高，IL-36β浓度最低。青少年患者IL-36α浓度高于成年患者，常年性变应原组和季节性变应原组的IL-36Ra水平存在差异。IL-36α水平与IL-36γ水平、IL-36γ水平与IL-36Ra水平、IL-36Ra水平与IL-38水平呈正相关，IL-36β水平分别与IL-36Ra和IL-38水平呈正相关。IL-36α水平与鼻塞症状的VAS评分呈正相关。IL-36β水平与眼部症状的总VAS评分以及眼痒和眼痛症状的VAS评分呈正相关。然而，IL-36家族所有细胞因子水平与SNOT-22评分、吸入性变应原阳性数量或变应原特异性免疫球蛋白E（sIgE）的最高阳性强度之间均无相关性。
外周血IL-36家族细胞因子在AR中起重要作用，IL-36α和IL-
