Department of Otolaryngology, First Affiliated Hospital to Harbin Medical University, Harbin, China.
Department of Otolaryngology, Second Affiliated Hospital to Harbin Medical University, Harbin, China.
Cytokine. 2020 Apr;128:154992. doi: 10.1016/j.cyto.2020.154992. Epub 2020 Jan 23.
T helper 17 (Th17) cell subsets, belongs to CD4+ T cell lineage, are proved to be closely related to pathophysiology of AR recently. The interleukin-36 (IL-36) had been reported to promote the up-regulation of Th17 cytokines in psoriasis. We investigated the regulation of Th17 inflammation by IL-36 family cytokines in allergic rhinitis (AR).
Twenty-one patients with AR and 20 healthy controls were enrolled. The expression of serum protein and mRNA of IL-36 family cytokines between AR and control group were detected and compared. Human peripheral blood mononuclear cells were purified and stimulated by IL-36 cytokines. The transcription factor and production of Th17 cytokines by Th17 cells were evaluated. Mouse model with AR was established to confirm the in vitro results.
The serum expression of IL-36 cytokines and Th17 cytokines (IL-17 and IL-23) of AR patients were up-regulated significantly compared with controls. The IL-36α promoted the differentiation and function of Th17 cells. The anti-IL-36α treatment could alleviate the Th17 response in AR mice, presented with alleviated symptoms, decreased infiltration of Th17 cells and down-regulated Th17 cytokines expression.
IL-36α was involved in the regulation of Th17 responses in allergic rhinitis.
辅助性 T 细胞 17（Th17）细胞亚群属于 CD4+T 细胞谱系，最近被证明与变应性鼻炎（AR）的病理生理学密切相关。白细胞介素-36（IL-36）已被报道可促进银屑病中 Th17 细胞因子的上调。我们研究了 IL-36 家族细胞因子在变应性鼻炎（AR）中对 Th17 炎症的调节作用。
纳入 21 例 AR 患者和 20 名健康对照者。检测并比较 AR 组和对照组血清中 IL-36 家族细胞因子的蛋白和 mRNA 表达。纯化人外周血单个核细胞，用 IL-36 细胞因子刺激。评估 Th17 细胞的转录因子和 Th17 细胞因子（IL-17 和 IL-23）的产生。建立 AR 小鼠模型以验证体外结果。
与对照组相比，AR 患者的血清 IL-36 细胞因子和 Th17 细胞因子（IL-17 和 IL-23）表达显著上调。IL-36α 促进了 Th17 细胞的分化和功能。抗-IL-36α 治疗可减轻 AR 小鼠的 Th17 反应，表现为症状减轻、Th17 细胞浸润减少和 Th17 细胞因子表达下调。
IL-36α 参与了变应性鼻炎中 Th17 反应的调节。
