Abdelrazek Samah M R, Connon Richard E, Sanchez Camilo, Atencio Benjamin, Mauduit Florian, Lehman Brendan, Hallett Sascha L, Atkinson Stephen D, Foott J Scott, Daniels Miles E
Department of Anatomy, Physiology and Cell Biology, University of California, Davis, Davis, CA 95616, USA.
Institute of Marine Sciences, University of California, Santa Cruz, Affiliated with Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Santa Cruz, CA 95060, USA.
Conserv Physiol. 2023 Aug 28;11(1):coad066. doi: 10.1093/conphys/coad066. eCollection 2023.
This study investigated how the deployment of juvenile Chinook salmon in ambient river conditions and the subsequent exposure to and infection by pathogens was associated with the changes in the expression of genes involved in immune system functioning, general stress and host development. Juvenile fish were deployed in sentinel cages for 21 days in the Sacramento River, CA, USA. Gill, kidney and intestinal tissue were sampled at 0, 7, 14 and 21 days post-deployment. Pathogen detection and host response were assessed by a combination of molecular and histopathological evaluation. Our findings showed that fish became infected by the parasites , and and to a lesser extent, the bacteria and -like organisms. Co-infection was common among sentinel fish. Expression of investigated genes was altered following deployment and was often associated with pathogen abundance. This study provides a foundation for future avenues of research investigating pathogens that affect out-migrating Chinook salmon in the Sacramento River, and offers crucial knowledge related to conservation efforts.
本研究调查了将幼年奇努克鲑鱼放养在自然河流环境中以及随后接触病原体并受其感染的情况，与参与免疫系统功能、一般应激反应和宿主发育的基因表达变化之间的关联。在美国加利福尼亚州萨克拉门托河的哨兵笼中放养幼鱼21天。在放养后0、7、14和21天采集鳃、肾脏和肠道组织样本。通过分子和组织病理学评估相结合的方式评估病原体检测和宿主反应。我们的研究结果表明，鱼类受到了寄生虫、和的感染，在较小程度上还受到了细菌和类生物体的感染。哨兵鱼中共同感染很常见。放养后，所研究基因的表达发生了改变，且通常与病原体丰度有关。本研究为未来研究影响萨克拉门托河洄游奇努克鲑鱼的病原体的研究途径奠定了基础，并提供了与保护工作相关的关键知识。
