Department of Microbiology, 226 Nash Hall, Oregon State University, Corvallis, OR, 97331, USA.
Department of Microbiology, 226 Nash Hall, Oregon State University, Corvallis, OR, 97331, USA; Institute of Parasitology, Biology Center of the Czech Academy of Sciences, České Budějovice, 37005, Czech Republic.
Dev Comp Immunol. 2021 Jan;114:103829. doi: 10.1016/j.dci.2020.103829. Epub 2020 Aug 23.
Two genotypes of the intestinal parasite Ceratonova shasta infect Oncorhynchus mykiss: genotype 0 results in a chronic infection with low mortality while genotype IIR causes disease with high mortality. We determined parasite load and the relative expression of six immune factors (IgT, IgM, IL-6, IL-8, IL-10, IFNG) in fish infected with either genotype over 29 days post-exposure. In genotype IIR infections the host responded with upregulation of inflammatory and regulatory cytokines. In contrast, genotype 0 infection did not elicit an inflammatory response and expression of IFNG and IL-10 was lower. Antibody expression was upregulated in both infections but appeared to have limited efficacy in the virulent genotype IIR infections. Histologically, in genotype 0 infections the parasite migrated through the tissue layers causing inflammation but minimal damage to the mucosal epithelium, which contrasts with the severe pathology found in genotype IIR infections.
两种肠道寄生虫 Ceratonova shasta 的基因型感染 Oncorhynchus mykiss：基因型 0 导致慢性感染，死亡率低，而基因型 II R 导致高死亡率的疾病。我们在感染后 29 天内确定了感染这两种基因型的鱼类的寄生虫负荷和六种免疫因子（IgT、IgM、IL-6、IL-8、IL-10、IFNG）的相对表达。在基因型 II R 感染中，宿主通过上调炎症和调节细胞因子做出反应。相比之下，基因型 0 感染不会引发炎症反应，IFNG 和 IL-10 的表达水平较低。两种感染都会上调抗体表达，但在毒力更强的基因型 II R 感染中似乎效果有限。组织学上，在基因型 0 感染中，寄生虫穿过组织层迁移，引起炎症，但对黏膜上皮的损伤很小，与基因型 II R 感染中发现的严重病理形成对比。
