Oluduro Anthonia O, Adesiyan Yetunde M, Omoboye Olumide O, Odeyemi Adebowale T
Department of Microbiology, Obafemi Awolowo University, Ile-Ife, 220005, Nigeria.
Landmark University SDG Groups 2 and 3; Department of Food Sciences and Microbiology, Landmark University, Omu-Aran, Kwara State, Nigeria.
AIMS Microbiol. 2023 Apr 23;9(3):402-418. doi: 10.3934/microbiol.2023021. eCollection 2023.
The presence of , a normal human flora on cellphones of different professionals in Ile-Ife was investigated with a view to determining their antibiotic susceptibility profile and nature of resistance and virulence genes. One hundred swab samples were collected aseptically from mobile phones of various users based on their profession. Surfaces of the mobile phones were swabbed and the streak plate method was used to isolate colonies showing characteristic golden yellow on mannitol salt agar plates. These isolates were further identified using standard microbiological methods. The antibiotic susceptibility of the isolates was determined using Kirby-Bauer's disk diffusion technique. Molecular detection of genes in some isolates was carried out by polymerase chain reaction technique. All the 36 isolates obtained in this study were 100% resistant to amoxicillin and augmentin; the isolates also displayed 55.6%, 44.4% and 41.7% resistance to ceftriazone, erythromycin and chloramphenicol, respectively. Based on resistance to oxacillin, prevalence of methicillin resistant (MRSA) was 11.1%. Only one was positive for plasmid analysis. gene was genetically confirmed in four (4) out of the 16 suspected phenotypic MRSA strains, gene was confirmed in all 28 isolates investigated, while there was no gene in the strains investigated. Mobile phones harbor multiple antibiotics resistant are responsible for important diseases in humans and could be difficult to manage with antibiotics thereby posing serious health risks.
对伊费地区不同职业人群手机上正常人类菌群的存在情况进行了调查，以确定其抗生素敏感性谱、耐药性和毒力基因的性质。根据职业，从不同用户的手机上无菌采集了100份拭子样本。对手机表面进行擦拭，采用划线平板法在甘露醇盐琼脂平板上分离出呈现特征性金黄色的菌落。使用标准微生物学方法对这些分离株进行进一步鉴定。采用 Kirby-Bauer 纸片扩散技术测定分离株的抗生素敏感性。通过聚合酶链反应技术对部分分离株的基因进行分子检测。本研究中获得的所有36株分离株对阿莫西林和奥格门汀均100%耐药；这些分离株对头孢曲松、红霉素和氯霉素的耐药率分别为55.6%、44.4%和41.7%。基于对苯唑西林的耐药性，耐甲氧西林金黄色葡萄球菌（MRSA）的流行率为11.1%。只有一株进行质粒分析呈阳性。在16株疑似表型MRSA菌株中有4株经基因确认存在mecA基因，在所研究的所有28株分离株中均确认存在tst基因，而在所研究的菌株中未发现sec基因。携带多种抗生素耐药金黄色葡萄球菌的手机是人类重要疾病的病因，且可能难以用抗生素治疗，从而带来严重的健康风险。
