Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.
Trop Biomed. 2023 Jun 1;40(2):194-198. doi: 10.47665/tb.40.2.011.
We have previously isolated a novel avian Orthobunyavirus, Kedah Fatal Kidney Syndrome (KFKS) virus from a broiler farm in Kedah, Malaysia in 2020 with a severe kidney lesion in chickens. The virus was designated as KFKS2_CS virus. Sequence analysis of partial nucleocapsid (N) and nonstructural (NSs) sequence of this virus showed the highest sequence identity with previous KFKS1 from Malaysia (100%) and 97% with a zoonotic Umbre (UMB) virus, which was reported to cause encephalitis in immunocompromised humans in India. Phylogenetic analysis revealed that this virus was clustered together with previous KFKS1 virus from Malaysia, UMB and Cristoli viruses. This study aimed to assess the zoonotic potential of this KFKS2_CS virus in vitro by determining its ability to inhibit the production of interferon (IFN) in human glioblastoma multiforme (GBM) brain cells using reverse-transcriptase polymerase reaction (RT-PCR). This virus blocked the production of interferon-a in this human brain cells. In conclusion, this KFKS2_CS virus may have a zoonotic potential and become a public health concern in the future.
我们曾于 2020 年在马来西亚吉打州的一家肉鸡场从病鸡中分离到一株新型禽正呼肠孤病毒，即吉打致命肾病综合征病毒。该病毒被命名为 KFKS2_CS 病毒。对该病毒部分核衣壳（N）和非结构（NSs）序列的测序结果表明，其与先前从马来西亚分离的 KFKS1 病毒（100%）和一种人畜共患的 Umb 病毒（UMB）（97%）具有最高的序列同一性，该病毒曾被报道在印度导致免疫功能低下人群的脑炎。系统进化分析显示，该病毒与先前从马来西亚、UMB 和 Cristoli 病毒分离的 KFKS1 病毒聚类在一起。本研究旨在通过检测该 KFKS2_CS 病毒在体外抑制人胶质母细胞瘤（GBM）脑细胞中干扰素（IFN）产生的能力，评估其潜在的人畜共患性，采用逆转录聚合酶链反应（RT-PCR）法。结果表明，该病毒可抑制人脑中干扰素-α的产生。综上，该 KFKS2_CS 病毒可能具有潜在的人畜共患性，未来或将成为公共卫生关注的焦点。
