Research Center for Engineering Technology of Kiwifruit, Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang 550025, China.
Teaching Experiment Farm, Guizhou University, Guiyang 550025, China.
J Agric Food Chem. 2023 Sep 13;71(36):13566-13576. doi: 10.1021/acs.jafc.3c02653. Epub 2023 Aug 31.
Kiwifruit canker is caused by pv and is one of the most destructive diseases of kiwifruit worldwide. Sulfur can improve the deposit of lignin in kiwifruit stems and induce disease resistance, but the action mechanism at the molecular level remains unclear. This omics-based study revealed that sulfur-induced S lignin synthesis contributes to disease resistance. Histological staining verified sulfur-enhanced total lignin deposition in kiwifruit stems. High-performance liquid chromatography and confocal Raman microscopy showed that sulfur-activated S lignin was mainly deposited in the cell corner. Metabolome and transcriptome analysis revealed that the levels of phenylpropanoid pathway S lignin precursors sinapic acid and sinapyl alcohol were significantly increased and 16 laccase genes were upregulated. Sulfur-induced resistance defense promoted elevated laccase activity by activating the laccase genes, participating in sinapic acid and sinapyl alcohol substance synthesis, and ultimately polymerizing S lignin at cell corner against kiwifruit canker disease.
猕猴桃溃疡病由 pv 引起，是世界范围内猕猴桃最具破坏性的疾病之一。硫可以改善猕猴桃茎木质素的沉积并诱导抗病性，但在分子水平上的作用机制尚不清楚。这项基于组学的研究表明，硫诱导的 S 木质素合成有助于抗病性。组织学染色验证了硫增强的猕猴桃茎中总木质素的沉积。高效液相色谱和共聚焦拉曼显微镜显示，硫激活的 S 木质素主要沉积在细胞角隅。代谢组学和转录组学分析表明，苯丙烷途径 S 木质素前体芥子酸和丁香醇的水平显著增加，16 个漆酶基因被上调。硫诱导的抗性防御通过激活漆酶基因、参与芥子酸和丁香醇物质的合成，以及最终在细胞角隅聚合 S 木质素来抵抗猕猴桃溃疡病，从而促进漆酶活性的升高。
