Hui Yuanyuan, Yang Ding, Wei Lusha, Pu Meixue, Mao Yazhou, Chen Xiaoxia, Wang Bini
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710119, China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710119, China; Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, China.
Food Chem. 2024 Jan 30;432:137271. doi: 10.1016/j.foodchem.2023.137271. Epub 2023 Aug 25.
A novel electrochemical aptasensor for the determination of streptomycin (STR) was constructed in this work. We prepared a palladium nanoparticles/titanium carbide/polyethyleneimine-functionalized nitrogen-doped carbon nanotube (Pd@TiC-PEI-NCNTs) composite via hydrothermal-assisted formic acid reduction and employed it as a substrate material for anchoring a NH-functionalized aptamer (Apt) to the surface of an electrode through the Pd-N bond. The Pd@TiC-PEI-NCNTs composite increased the electrochemically active surface area and conductivity of the electrode, achieving substantial signal amplification (1.87 times). The constructed aptasensor demonstrated accurate STR detection via specific recognition between the Apt and STR, with a detection range and limit of 0.01-700 and 0.003 nmol/L, respectively. The aptasensor also exhibited excellent selectivity, stability, reproducibility, practicability and shorter assay times (60 min). Furthermore, molecular docking and molecular dynamic also elucidated the binding mechanism between the aptamer and STR molecules. Overall, this method is expected to be a helpful tool for detecting STR in food.
在本工作中构建了一种用于测定链霉素（STR）的新型电化学适配体传感器。我们通过水热辅助甲酸还原法制备了钯纳米颗粒/碳化钛/聚乙烯亚胺功能化的氮掺杂碳纳米管（Pd@TiC-PEI-NCNTs）复合材料，并将其用作基底材料，通过Pd-N键将NH功能化的适配体（Apt）锚定在电极表面。Pd@TiC-PEI-NCNTs复合材料增加了电极的电化学活性表面积和导电性，实现了显著的信号放大（1.87倍）。构建的适配体传感器通过Apt与STR之间的特异性识别实现了对STR的准确检测，检测范围和检测限分别为0.01 - 700和0.003 nmol/L。该适配体传感器还表现出优异的选择性、稳定性、重现性、实用性和较短的检测时间（60分钟）。此外，分子对接和分子动力学也阐明了适配体与STR分子之间的结合机制。总体而言，该方法有望成为检测食品中STR的有用工具。
