School of Public Health, Health Science Center, Ningbo University, Ningbo, Zhejiang, China.
Institute of Geriatrics, The First Affiliated Hospital of Medical School, Ningbo University, Ningbo, Zhejiang, China.
Eur J Clin Nutr. 2024 Jan;78(1):19-26. doi: 10.1038/s41430-023-01339-y. Epub 2023 Aug 31.
To evaluate the causal effect of plasma omega-3 polyunsaturated fatty acids (PUFAs) on sarcopenia-related traits (lean mass, grip strength and walking pace) utilizing two-sample Mendelian randomization (MR) approach.
Based on genome-wide association study (GWAS) summary statistics, we performed two-sample MR applying the inverse variance weighted (IVW) as the primary method, supplemented with four additional sensitivity analyses. Furthermore, multivariable MR (MVMR) was applied to assess these associations independent of alcohol drinking, type 2 diabetes (T2D), triglycerides (TG), estimated glomerular filtration rate (eGFR) and C-reactive protein (CRP).
In univariable MR, the IVW analysis suggested no significant causal effect of genetically determined plasma omega-3 PUFAs on fat-free mass (right leg: β = 0.01, 95% CI = -0.02 to 0.05, P = 0.375; left leg: β = 0.01, 95% CI = -0.02 to 0.04, P = 0.446; right arm: β = 0.01, 95% CI = -0.02 to 0.05, P = 0.376; left arm: β = 0.01, 95% CI = -0.02 to 0.04, P = 0.384; trunk:β = 0.02, 95% CI = -0.02 to 0.06, P = 0.283; whole: β = 0.01, 95% CI = -0.03 to 0.04, P = 0.631), grip strength (right hand: β = -0.01, 95% CI = -0.03 to 0.01, P = 0.387; left hand: β = -0.01, 95% CI = -0.02 to 0.01, P = 0.553) and walking pace (β = 0.00, 95% CI = -0.01 to 0.02, P = 0.575), and sensitive analysis generated similar non-significant results. Furthermore, the MVMR revealed no independent causal association.
Genetically determined plasma omega-3 PUFAs have no causal effect on sarcopenia-related traits.
利用两样本 Mendelian 随机化（MR）方法评估血浆 ω-3 多不饱和脂肪酸（PUFAs）对与肌肉减少症相关的特征（瘦体重、握力和行走速度）的因果效应。
基于全基因组关联研究（GWAS）汇总统计数据，我们应用逆方差加权（IVW）作为主要方法进行两样本 MR 分析，并辅以四种额外的敏感性分析。此外，还应用多变量 MR（MVMR）来评估这些关联，同时控制饮酒、2 型糖尿病（T2D）、甘油三酯（TG）、估计肾小球滤过率（eGFR）和 C 反应蛋白（CRP）的影响。
在单变量 MR 中，IVW 分析表明，遗传确定的血浆 ω-3 PUFAs 对无脂肪质量（右腿：β=0.01，95%置信区间=-0.02 至 0.05，P=0.375；左腿：β=0.01，95%置信区间=-0.02 至 0.04，P=0.446；右臂：β=0.01，95%置信区间=-0.02 至 0.05，P=0.376；左臂：β=0.01，95%置信区间=-0.02 至 0.04，P=0.384；躯干：β=0.02，95%置信区间=-0.02 至 0.06，P=0.283；全身：β=0.01，95%置信区间=-0.03 至 0.04，P=0.631）、握力（右手：β=-0.01，95%置信区间=-0.03 至 0.01，P=0.387；左手：β=-0.01，95%置信区间=-0.02 至 0.01，P=0.553）和行走速度（β=0.00，95%置信区间=-0.01 至 0.02，P=0.575）没有因果效应，敏感性分析也得到了相似的非显著结果。此外，MVMR 也未揭示出独立的因果关联。
遗传确定的血浆 ω-3 PUFAs 对与肌肉减少症相关的特征没有因果影响。
