沃尔巴克氏体 wMel 株介导的登革病毒经埃及伊蚊垂直传播至其后代的影响。
Wolbachia wMel strain-mediated effects on dengue virus vertical transmission from Aedes aegypti to their offspring.
机构信息
Oxford University Clinical Research Unit, Wellcome Trust Africa Asia Programme, District 5, Ho Chi Minh City, Vietnam.
Hospital for Tropical Diseases, District 5, Ho Chi Minh City, Vietnam.
出版信息
Parasit Vectors. 2023 Aug 31;16(1):308. doi: 10.1186/s13071-023-05921-y.
BACKGROUND
Dengue virus serotypes (DENV-1 to -4) can be transmitted vertically in Aedes aegpti mosquitoes. Whether infection with the wMel strain of the endosymbiont Wolbachia can reduce the incidence of vertical transmission of DENV from infected females to their offspring is not well understood.
METHODS
A laboratory colony of Vietnamese Ae. aegypti, both with and without wMel infection, were infected with DENV-1 by intrathoracic injection (IT) to estimate the rate of vertical transmission (VT) of the virus. VT in the DENV-infected mosquitoes was calculated via the infection rate estimation from mosquito pool data using maximum likelihood estimation (MLE).
RESULTS
In 6047 F1 Vietnamese wild-type Ae. aegypti, the MLE of DENV-1 infection was 1.49 per 1000 mosquitoes (95% confidence interval [CI] 0.73-2.74). In 5500 wMel-infected Ae. aegypti, the MLE infection rate was 0 (95% CI 0-0.69). The VT rates between mosquito lines showed a statistically significant difference.
CONCLUSIONS
The results reinforce the view that VT is a rare event in wild-type mosquitoes and that infection with wMel is effective in reducing VT.
背景
登革病毒血清型（DENV-1 至 DENV-4）可在埃及伊蚊中垂直传播。感染共生菌沃尔巴克氏体的 wMel 株是否能降低感染雌蚊垂直传播登革病毒（DENV）至其后代的发生率，目前尚不清楚。
方法
利用胸内注射（IT）的方法，对带有和不带有 wMel 感染的越南埃及伊蚊实验室种群进行 DENV-1 感染，以估计病毒的垂直传播（VT）率。通过使用最大似然估计法（MLE）从蚊子群体数据中计算病毒感染率，从而计算 DENV 感染蚊子的 VT。
结果
在 6047 只 F1 越南野生型埃及伊蚊中，DENV-1 感染的 MLE 为每 1000 只蚊子 1.49（95%置信区间[CI]0.73-2.74）。在 5500 只感染 wMel 的埃及伊蚊中，感染率的 MLE 为 0（95%CI0-0.69）。蚊系之间的 VT 率存在统计学显著差异。
结论
结果强化了 VT 是野生型蚊子中罕见事件的观点，并且感染 wMel 可有效降低 VT。
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