Omotani Sachiko, Murakami Kanaha, Naka Arisa, Hatsuda Yasutoshi, Myotoku Michiaki
Faculty of Pharmacy, Osaka Ohtani University, 3-11-1, Nishikiori-Kita, Tondabayashi, Osaka, 584-8540, Japan.
J Pharm Health Care Sci. 2023 Sep 1;9(1):27. doi: 10.1186/s40780-023-00297-8.
Enteral nutritional supplements are used in many medical facilities and home care, but require appropriate management because they are nutrient-rich products. Recently, infection control methods for Ready To Hang (RTH) preparations, which are widely used and are expected to reduce the risk of infection, have not been established in Japan and are dependent on caregivers. Therefore, we evaluated the difference in the growth of microorganisms depending on the type of enteral nutrients following contamination with microorganisms.
Nine types of enteral nutrition were used. Escherichia coli (E. coli) W3110, Serratia marcescens (S. marcescens) NBRC3046, and Candida albicans (C. albicans) IFM61197 were used as test bacteria. The bacterial solution was added to the enteral nutritional supplement, adjusted, and the number of bacteria was measured at 0, 4, 8, and 24 h after the addition of the bacterial solution at 25 °C and in the dark.
E. coli and S. marcescens grew in RACOL-NF SemiSolid for Enteral Use, Hine Jerry AQUA, and Mermed Plus over a 24-h period; however, a decrease was observed for other enteral nutrition products. In contrast, C. albicans grew in all enteral nutrition products.
Because the viscosity and calorie content vary among enteral nutrition preparations in which growth was observed, we found that pH had the greatest effect on the differences in bacterial growth. Nonetheless, C. albicans growth occurred in all nine types of enteral nutrients, indicating that unlike bacteria, its growth was independent of pH. If semi-solid enteral nutrients are contaminated with microorganisms for any reason, microorganisms will grow, so appropriate infection control is necessary to prevent infection.
肠内营养补充剂在许多医疗机构和家庭护理中都有使用，但由于它们是营养丰富的产品，需要进行适当管理。最近，随时悬挂式（RTH）制剂的感染控制方法在日本尚未确立，且依赖于护理人员，而这种制剂被广泛使用并有望降低感染风险。因此，我们评估了微生物污染后，不同类型肠内营养制剂中微生物生长的差异。
使用了九种类型的肠内营养制剂。将大肠杆菌（E. coli）W3110、粘质沙雷氏菌（S. marcescens）NBRC3046和白色念珠菌（C. albicans）IFM61197用作测试细菌。将细菌溶液添加到肠内营养补充剂中，进行调整，并在25°C黑暗条件下添加细菌溶液后的0、4、8和24小时测量细菌数量。
在24小时内，大肠杆菌和粘质沙雷氏菌在肠内用RACOL-NF半固体、Hine Jerry AQUA和Mermed Plus中生长；然而，在其他肠内营养产品中观察到细菌数量减少。相比之下，白色念珠菌在所有肠内营养产品中均生长。
由于观察到生长的肠内营养制剂的粘度和热量含量各不相同，我们发现pH对细菌生长差异的影响最大。尽管如此，白色念珠菌在所有九种肠内营养制剂中均生长，这表明与细菌不同，其生长与pH无关。如果半固体肠内营养制剂因任何原因被微生物污染，微生物将会生长，因此需要进行适当的感染控制以预防感染。
