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对……的转录组测序揭示了……中与茎相关的基因表达模式。 (你提供的原文信息不完整,我只能根据已有内容尽量翻译准确,具体完整准确的翻译需补充完整原文)

Transcriptome Sequencing of Reveals Shoot-Related Expression Patterns of Genes in .

作者信息

Wang Xuxia, Huang Xing, Chen Lisha, Xie Zhouli, Tan Shibei, Qin Xu, Chen Tao, Huang Yanlei, Xi Jingen, Chen Helong, Yi Kexian

机构信息

Urban Construction College, Wuchang Shouyi University, Wuhan 430064, China.

Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.

出版信息

Plants (Basel). 2023 May 18;12(10):2020. doi: 10.3390/plants12102020.

Abstract

species are widely planted for fiber production. However, the molecular basis of agave fiber development has not been well understood. In this study, we performed a transcriptomic analysis in , a well-known variety with high-quality fiber production. Approximately 43.87 million clean reads were obtained using Illumina sequencing. The de novo assembly produced 66,746 unigrams, 54% of which were annotated in a public database. In the Nr database, 21,490 unigenes of were shown to be most closely related to . Nine orthologs with full coding regions were obtained, which were named , , , , , , , and . The maximum likelihood phylogenetic tree revealed the species-specific expansion of genes in , rice and agave. The expression analysis suggested the negative correlation between the expression of genes and the leaf growth rate, except . Moreover, genes were differentially affected by abiotic and biotic stresses. Notably, expression level was highly upgraded after the infection of . Nutrient deficiency also influent genes expression. Together, our research will benefit future studies related to fiber development, disease resistance and nutrient usage in agave.

摘要

龙舌兰属植物被广泛种植用于纤维生产。然而,龙舌兰纤维发育的分子基础尚未得到很好的理解。在本研究中,我们对一种以生产高质量纤维而闻名的品种进行了转录组分析。使用Illumina测序获得了约4387万个clean reads。从头组装产生了66746个单基因,其中54%在公共数据库中得到注释。在Nr数据库中,该品种的21490个单基因显示与[相关物种]关系最为密切。获得了9个具有完整编码区的直系同源基因,分别命名为[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]、[基因名称5]、[基因名称6]、[基因名称7]、[基因名称8]和[基因名称9]。最大似然系统发育树揭示了[相关基因]在[相关物种]、水稻和龙舌兰中的物种特异性扩张。表达分析表明,除了[某个基因]外,[相关基因]的表达与叶片生长速率呈负相关。此外,[相关基因]受到非生物和生物胁迫的不同影响。值得注意的是,在[某种病原体]感染后,[某个基因]的表达水平大幅提高。营养缺乏也影响[相关基因]的表达。总之,我们的研究将有助于未来关于龙舌兰纤维发育、抗病性和养分利用的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcde/10222593/91626716b586/plants-12-02020-g001.jpg

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