Tan Shibei, Liang Yanqiong, Huang Yanlei, Xi Jingen, Huang Xing, Yang Xiaohan, Yi Kexian
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2022 May 27;11(11):1434. doi: 10.3390/plants11111434.
species are widely cultivated crassulacean acid metabolism (CAM) plants for alcoholic beverages, food and fiber production. Among these, the hybrid H11648 (( × ) × ) is the main cultivar for sisal fiber in the tropical areas of Brazil, China, and African countries. The plants of hybrid H11648 have a long life cycle and large leaves, which require a huge amount of nitrogen nutrient. However, the molecular basis of nitrogen transport and allocation has not been well understood in agave. In this study, we identified 19 /() genes (called ) with full-length coding sequences in hybrid H11648. Our analysis of gene expression in various types of tissues revealed the tissue-specific expression pattern of . We further examined their expression patterns at different leaf developmental stages, under abiotic/biotic stresses and nutrient deficiency. The results reveal several candidate regulators in the agave family, including // We first characterized the genes in agave based on published leaf transcriptome datasets and emphasized their potential functions. The study will benefit future studies related to nitrogen nutrient in agave.
龙舌兰属植物是广泛种植的用于生产酒精饮料、食品和纤维的景天酸代谢（CAM）植物。其中，杂交种H11648（（×）×）是巴西、中国和非洲国家热带地区剑麻纤维的主要栽培品种。杂交种H11648的植株生命周期长且叶片大，需要大量的氮养分。然而，龙舌兰中氮转运和分配的分子基础尚未得到很好的理解。在本研究中，我们在杂交种H11648中鉴定出19个具有全长编码序列的/()基因（称为）。我们对不同类型组织中基因表达的分析揭示了的组织特异性表达模式。我们进一步研究了它们在不同叶片发育阶段、非生物/生物胁迫和养分缺乏条件下的表达模式。结果揭示了龙舌兰属家族中的几个候选调节因子，包括//我们首先基于已发表的叶片转录组数据集对龙舌兰中的基因进行了表征，并强调了它们的潜在功能。该研究将有助于未来与龙舌兰中氮养分相关的研究。
