Degtyaryov Evgeny, Pigolev Alexey, Miroshnichenko Dmitry, Frolov Andrej, Basnet Adi Ti, Gorbach Daria, Leonova Tatiana, Pushin Alexander S, Alekseeva Valeriya, Dolgov Sergey, Savchenko Tatyana
Institute of Basic Biological Problems, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia.
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia.
Plants (Basel). 2023 May 22;12(10):2050. doi: 10.3390/plants12102050.
12-Oxophytodienoate reductase is the enzyme involved in the biosynthesis of phytohormone jasmonates, which are considered to be the major regulators of plant tolerance to biotic challenges, especially necrotrophic pathogens. However, we observe compromised tolerance to the necrotrophic fungal pathogen in transgenic hexaploid bread wheat and tetraploid emmer wheat plants overexpressing gene from , while in Arabidopsis plants themselves, endogenously produced and exogenously applied jasmonates exert a strong protective effect against . Exogenous application of methyl jasmonate on hexaploid and tetraploid wheat leaves suppresses tolerance to and induces the formation of chlorotic damages. Exogenous treatment with methyl jasmonate in concentrations of 100 µM and higher causes leaf yellowing even in the absence of the pathogen, in agreement with findings on the role of jasmonates in the regulation of leaf senescence. Thereby, the present study demonstrates the negative role of the jasmonate system in hexaploid and tetraploid wheat tolerance to and reveals previously unknown jasmonate-mediated responses.
12-氧代植物二烯酸还原酶是参与植物激素茉莉酸生物合成的酶，茉莉酸被认为是植物对生物胁迫尤其是坏死营养型病原体耐受性的主要调节因子。然而，我们观察到，在过表达来自[具体来源未提及]基因的转基因六倍体面包小麦和四倍体二粒小麦植株中，对坏死营养型真菌病原体的耐受性受损，而在拟南芥植株本身中，内源性产生和外源性施加的茉莉酸对[具体病原体未提及]具有很强的保护作用。在六倍体和四倍体小麦叶片上外源性施加茉莉酸甲酯会抑制对[具体病原体未提及]的耐受性，并诱导形成褪绿损伤。即使在没有病原体的情况下，以100 µM及更高浓度的茉莉酸甲酯进行外源性处理也会导致叶片发黄，这与茉莉酸在叶片衰老调节中的作用的研究结果一致。因此，本研究证明了茉莉酸系统在六倍体和四倍体小麦对[具体病原体未提及]耐受性中的负面作用，并揭示了此前未知的茉莉酸介导的反应。
