东亚石竹科植物的植物蜕皮甾体类化合物。

Phytoecdysteroids of the East Asian Caryophyllaceae.

作者信息

Novozhilova Elena, Rybin Viacheslav, Gorovoy Petr, Gavrilenko Irina, Doudkin Roman

机构信息

Laboratory of Plant Chemotaxonomy, G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.

Laboratory of Comparative Biochemistry, A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.

出版信息

Pharmacogn Mag. 2015 May;11(Suppl 1):S225-30. doi: 10.4103/0973-1296.157746.

DOI:10.4103/0973-1296.157746

PMID:26109770

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4461964/

Abstract

BACKGROUND

Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae.

MATERIALS AND METHODS

Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution mass spectrometry was performed on Shimadzu LCMS-IT-TOF (Japan) system equipped with a LC-20A Prominence liquid chromatograph, a photodiode array detector SPD-M20A and ion-trap/time-of-flight mass spectrometer.

RESULTS

New sources of phytoecdysteroids: Melandrium sachalinense and Melandrium firmum have been revealed. It is the 1(st) time that two has been identified in M. sachalinense and M. firmum; 1 in the species: Lychnis fulgens, Silene repens, Silene foliosa, Silene stenophylla, Silene jenisseensis and M. sachalinense; 3 in Lychnis cognata; 4 in L. fulgens, S. stenophylla and S. jenisseensis (the tribe Lychnideae, the subfamily Caryophylloideae). Ecdysteroid-negative taxa are Spergularia rubra of the tribe Sperguleae; species of the genera Minuartia, Honckenya, Eremogone, Arenaria, Moehringia, Pseudostellaria, Fimbripetalum, Stellaria and Cerastium of the tribe Alsineae; Scleranthus annuus of the tribe Sclerantheae, as well as the East Asian representatives of the genera Gypsophila, Psammophiliela, Dianthus and Saponaria of the tribe Diantheae; Oberna and Agrostemma of the tribe Lychnideae.

CONCLUSION

This investigation shows the most promising sources of ecdysteriods are species of genera Silene and Lychnis.

摘要

背景

对东亚石竹科64个物种中的整合甾酮A（1）、20-羟基蜕皮激素（2）、蜕皮激素（3）、2-脱氧-20-羟基蜕皮激素（4）的存在情况进行了分析。

材料与方法

采用高效液相色谱法（HPLC）测定蜕皮甾类含量。在配备了LC-20A Prominence液相色谱仪、光电二极管阵列检测器SPD-M20A和离子阱/飞行时间质谱仪的岛津LCMS-IT-TOF（日本）系统上进行了带有高分辨率质谱的HPLC分析。

结果

发现了植物蜕皮甾类的新来源：库页蝇子草和坚挺蝇子草。这是首次在库页蝇子草和坚挺蝇子草中鉴定出两种；在以下物种中鉴定出1种：裂叶剪秋罗、匐生蝇子草、叶状蝇子草、窄叶蝇子草、黑龙江蝇子草和库页蝇子草；在浅裂剪秋罗中鉴定出3种；在裂叶剪秋罗、窄叶蝇子草和黑龙江蝇子草（剪秋罗族，石竹亚科）中鉴定出4种。蜕皮甾类阴性类群有小拟漆姑草族的红拟漆姑草；繁缕族的米努草属、薄蒴草属、旱麦草属、砂苋属、卷耳属、孩儿参属、瓣蕊唐松草属、繁缕属和卷耳属的物种；粟米草族的一年生粟米草，以及石竹族的丝石竹属、拟硬叶丝石竹属、石竹属和肥皂草属的东亚代表物种；剪秋罗族的欧剪秋罗属和麦仙翁属。